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PMID: 6296450 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Processing of the encephalomyocarditis virus capsid precursor protein studied in rabbit reticulocyte lysates incubated with N-formyl-[35S]methionine-tRNAfMet.

Journal of virology ·Vol. 45 ·No. 1 ·1983-01-00 ·Pages 439-41

Campbell EA, Jackson RJ

Abstract

Translation of encephalomyocarditis virus RNA in rabbit reticulocyte lysates in the presence of N-formyl-[(35)S]methionine-tRNA(f) (Met) revealed that a small polypeptide is cleaved from the N-terminus of the capsid protein precursor, preA, by virus-coded protease activity. Therefore, this N-terminal segment comprising the translation initiation site is not conserved in any of the mature capsid proteins.

MeSH Terms
Animals Capsid/metabolism Encephalomyocarditis virus/metabolism Peptide Hydrolases/metabolism Protein Precursors/metabolism Protein Processing, Post-Translational RNA, Transfer, Amino Acyl RNA, Transfer, Met Rabbits Reticulocytes Sulfur Radioisotopes Viral Proteins/metabolism
Chemicals
Protein Precursors RNA, Transfer, Amino Acyl RNA, Transfer, Met Sulfur Radioisotopes Viral Proteins tRNA, formylmethionine- Peptide Hydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Campbell E A
Jackson R J
References (9)
9 references, click to expand
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    Anal Biochem. 1972 Jul;48(1):202-16 PMID: 4557421
  2. Characterization of the large picornaviral polypeptides produced in the presence of zinc ion.
    J Virol. 1974 Aug;14(2):282-91 PMID: 4367904
  3. Identification of a viral protein involved in post-translational maturation of the encephalomyocarditis virus capsid precursor.
    J Virol. 1975 Apr;15(4):918-28 PMID: 163926
  4. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  5. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  6. Complete translation of encephalomyocarditis virus RNA and faithful cleavage of virus-specific proteins in a cell-free system from Krebs-2 cells.
    FEBS Lett. 1978 Mar 1;87(1):7-11 PMID: 204520
  7. Translation of encephalomyocarditis virus RNA in vitro yields an active proteolytic processing enzyme.
    Eur J Biochem. 1978 Apr 17;85(2):457-62 PMID: 206439
  8. Translation of encephalomyocarditis virus RNA in reticulocyte lysates: kinetic analysis of the formation of virion proteins and a protein required for processing.
    J Virol. 1979 May;30(2):472-80 PMID: 224211
  9. Picornaviral structure and assembly.
    Microbiol Rev. 1981 Jun;45(2):287-315 PMID: 7022155
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.45.1.439-441.1983
Published
1983-01-00
Pages
439-41
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256426
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.45.1.439-441.1983 PMC Full Text View journal details
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