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PMID: 6298179 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Plasmid-borne sulfonamide resistance determinants studied by restriction enzyme analysis.

Journal of bacteriology ·Vol. 153 ·No. 3 ·1983-03-00 ·Pages 1228-37

Swedberg G, Sköld O

Abstract

The relationship between sulfonamide resistance genes carried on different plasmids was investigated by restriction enzyme analysis and DNA-DNA hybridization. The results showed that sulfonamide resistance mediated by different plasmids is determined by the production of at least two different types of drug-resistant dihydropteroate synthase. Plasmids pGS01, pGS02, and R22259, found in bacteria isolated from patients in Swedish hospitals, contained identical sulfonamide resistance genes, which were also identical to those of plasmids R1, R100, R6, and R388. These latter plasmids, which have been well studied in different laboratories, were originally from clinical isolates from different parts of the world. Two other clinically isolated plasmids, pGS04 and pGS05, were shown to contain sulfonamide resistance determinants of a completely different type.

MeSH Terms

DNA Restriction Enzymes/metabolism DNA, Bacterial/analysis Deoxyribonuclease BamHI Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Drug Resistance, Microbial Electrophoresis, Agar Gel Nucleic Acid Hybridization Plasmids Sulfonamides/pharmacology

Chemicals

DNA, Bacterial Sulfonamides DNA Restriction Enzymes Deoxyribonuclease BamHI Deoxyribonuclease EcoRI Deoxyribonuclease HindIII

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Swedberg G

Sköld O

References (10)

10 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.153.3.1228-1237.1983

Published

1983-03-00

Pages

1228-37

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC221767

Subset

External Links

PubMed source DOI: 10.1128/jb.153.3.1228-1237.1983 PMC Full Text View journal details

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Plasmid-borne sulfonamide resistance determinants studied by restriction enzyme analysis.

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