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PMID: 6298469 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transcription of mouse mammary tumor virus: identification of a candidate mRNA for the long terminal repeat gene product.

Journal of virology ·Vol. 46 ·No. 1 ·1983-04-00 ·Pages 42-9

Wheeler DA, Butel JS, Medina D, Cardiff RD, Hager GL

Abstract

We have examined an assortment of preneoplastic and neoplastic mouse mammary tissues for the presence of an mRNA which could encode the putative long terminal repeat gene product of mouse mammary tumor virus. We report here the detection of a novel mouse mammary tumor virus-specific, polyadenylic acid-containing transcript in certain preneoplastic and neoplastic mammary tissue of BALB/c mice. The molecule is 1.6 kilobases in length and contains sequences from the transcriptional leader and the U3 region of the proviral DNA. The upstream terminus of the 3' information lies 75 to 80 nucleotides from the beginning of the long terminal repeat open reading frame, in close proximity to a consensus splice acceptor in the DNA. The transcript was detected in hormonally or chemically induced neoplastic, preneoplastic, and lactating mammary tissue of BALB/c mice, but not in preneoplastic or tumor tissue induced by exogenous viruses in any strain of mice examined. This implies that the RNA we observed is transcribed from an endogenous provirus template.

MeSH Terms
Animals DNA, Viral/analysis Female Genes, Viral Mammary Neoplasms, Experimental/microbiology Mammary Tumor Virus, Mouse/genetics Mice Mice, Inbred Strains Nucleic Acid Hybridization RNA Splicing RNA, Messenger/analysis RNA, Viral/analysis Repetitive Sequences, Nucleic Acid Tissue Distribution Transcription, Genetic
Chemicals
DNA, Viral RNA, Messenger RNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wheeler D A
Butel J S
Medina D
Cardiff R D
Hager G L
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39 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.46.1.42-49.1983
Published
1983-04-00
Pages
42-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255091
Subset
IM
Grants
NCI NIH HHS · N0I-CP9-1020 · United States
External Links
PubMed source DOI: 10.1128/JVI.46.1.42-49.1983 PMC Full Text View journal details
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