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PMID: 6298724 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Structure and transcription of the nopaline synthase gene region of T-DNA.

Nucleic acids research ·Vol. 11 ·No. 2 ·1983-01-25 ·Pages 369-85

Bevan M, Barnes WM, Chilton MD

Abstract

We present the DNA sequence and plant-tumor transcription pattern of some 2400 base pairs from the right border region of pTi T37 DNA from the virulent Agrobacterium tumefaciens strain T37. This region includes the entire transcription unit encompassing the nopaline synthase gene, together with parts of other transcription units. The strategy used to determine the sequence also produced two opposing series of defined, asymmetric deletions across the target DNA region, some of which may serve future purposes in the exploitation of this sequence, which is known to be expressed in a wide variety of host plant tissues.

MeSH Terms
Amino Acid Oxidoreductases/genetics Amino Acid Sequence Base Sequence DNA Restriction Enzymes DNA, Bacterial/genetics Genes Nucleic Acid Hybridization Plant Tumors Plasmids Rhizobium/enzymology Transcription, Genetic
Chemicals
DNA, Bacterial Amino Acid Oxidoreductases nopaline synthase DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bevan M
Barnes W M
Chilton M D
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40 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.2.369
Published
1983-01-25
Pages
369-85
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325720
Subset
IM
Grants
NIGMS NIH HHS · GM24956 · United States
Databases
GENBANK
J01541, V00087
External Links
PubMed source DOI: 10.1093/nar/11.2.369 PMC Full Text View journal details
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