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PMID: 6298739 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Poliovirus type 3: molecular cloning of the genome and nucleotide sequence of the region encoding the protease and polymerase proteins.

Nucleic acids research ·Vol. 11 ·No. 5 ·1983-03-11 ·Pages 1267-81

Cann AJ, Stanway G, Hauptmann R, Minor PD, Schild GC, Clarke LD, Mountford RC, Almond JW

Abstract

Overlapping cDNA clones representing the entire genome of poliovirus type 3 have been prepared in E. coli by two separate methods. Cloning of RNA . cDNA hybrids produced a more comprehensive set of clones with generally larger cDNA inserts than cloning of double - stranded cDNA. A restriction map of the entire genome and the nucleotide sequence of 2003 bases from the 3' terminus, comprising the region encoding the protease and polymerase proteins, are presented.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Viral Nucleic Acid Hybridization Nucleotidyltransferases/genetics Peptide Hydrolases/genetics Plasmids Poliovirus/enzymology,genetics
Chemicals
DNA Nucleotidyltransferases DNA Restriction Enzymes Peptide Hydrolases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Cann A J
Stanway G
Hauptmann R
Minor P D
Schild G C
Clarke L D
Mountford R C
Almond J W
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.5.1267
Published
1983-03-11
Pages
1267-81
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325795
Subset
IM
Databases
GENBANK
J02283
External Links
PubMed source DOI: 10.1093/nar/11.5.1267 PMC Full Text View journal details
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