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PMID: 6299270 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Interaction of beta-iodopenicillanate with the beta-lactamases of Streptomyces albus G and Actinomadura R39.

The Biochemical journal ·Vol. 207 ·No. 3 ·1982-12-01 ·Pages 437-44

Frère JM, Dormans C, Duyckaerts C, De Graeve J

Abstract

The beta-lactamases of Streptomyces albus G and Actinomadura R39 are inactivated by beta-iodopenicillanate. However, in contrast with the beta-lactamase I from Bacillus cereus, they also efficiently catalyse the hydrolysis of the inactivator; with the S. albus G enzyme, kcat. is larger than 25s-1 and the number of turnovers before inactivation is 515. With the A. R39 enzyme, kcat. is larger than 50s-1 and the number of turnovers before inactivation is 80. After hydrolysis of the beta-lactam amide bond, the product rearranges into 2.3-dihydro-2,2-dimethyl-1,4-thiazine-3,6-dicarboxylate, which exhibits an absorption maximum at 305 nm.

MeSH Terms
Actinomycetaceae/enzymology Hydrolysis Kinetics Mass Spectrometry Models, Biological Penicillanic Acid/metabolism Streptomyces/enzymology beta-Lactamase Inhibitors
Chemicals
beta-Lactamase Inhibitors Penicillanic Acid 6-iodopenicillanic acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Frère J M
Dormans C
Duyckaerts C
De Graeve J
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20 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2070437
Published
1982-12-01
Pages
437-44
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1153883
Subset
IM
Grants
NIAID NIH HHS · 2 R01 AI 13364-05 · United States
External Links
PubMed source DOI: 10.1042/bj2070437 PMC Full Text View journal details
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