Home LiteratureArticle Details
PMID: 6300459 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Use of monoclonal antibodies against two 75,000-molecular-weight glycoproteins specified by herpes simplex virus type 2 in glycoprotein identification and gene mapping.

Journal of virology ·Vol. 45 ·No. 3 ·1983-03-00 ·Pages 1223-7

Para MF, Zezulak KM, Conley AJ, Weinberger M, Snitzer K, Spear PG

Abstract

We produced two monoclonal antibodies that precipitate different glycoproteins of similar apparent molecular weight (70,000 to 80,000) from extracts of cells infected with herpes simplex virus type 2. Evidence is presented that one of these glycoproteins is the previously characterized glycoprotein gE, whereas the other maps to a region of the herpes simplex virus type 2 genome collinear with the region in herpes simplex virus type 1 DNA that encodes gC.

MeSH Terms
Antibodies, Monoclonal/immunology Antibodies, Viral/immunology Glycoproteins/genetics,immunology Simplexvirus/genetics,immunology Viral Proteins/genetics,immunology
Chemicals
Antibodies, Monoclonal Antibodies, Viral Glycoproteins Viral Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Para M F
Zezulak K M
Conley A J
Weinberger M
Snitzer K
Spear P G
References (22)
22 references, click to expand
  1. Glycoproteins specified by herpes simplex virus type 1: their synthesis, processing and antigenic relatedness to HSV -2 glycoproteins.
    IARC Sci Publ. 1975;(11 Pt 1):49-61 PMID: 65316
  2. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  3. Anatomy of herpes simplex virus DNA. IX. Apparent exclusion of some parental DNA arrangements in the generation of intertypic (HSV-1 X HSV-2) recombinants.
    J Virol. 1977 Oct;24(1):231-48 PMID: 198577
  4. Identification of a common antigen of herpes simplex virus bovine herpes mammillitis virus, and B virus.
    J Virol. 1978 Jun;26(3):712-7 PMID: 78988
  5. A better cell line for making hybridomas secreting specific antibodies.
    Nature. 1978 Nov 16;276(5685):269-70 PMID: 714156
  6. Physical mapping of herpes simplex virus-induced polypeptides.
    J Virol. 1978 Nov;28(2):624-42 PMID: 214583
  7. Molecular genetics of herpes simplex virus. II. Mapping of the major viral glycoproteins and of the genetic loci specifying the social behavior of infected cells.
    J Virol. 1979 Feb;29(2):677-97 PMID: 219254
  8. Membrane proteins specified by herpes simplex viruses. V. Identification of an Fc-binding glycoprotein.
    J Virol. 1979 Dec;32(3):779-89 PMID: 229267
  9. Preparation and characterization of specific antisera to individual glycoprotein antigens comprising the major glycoprotein region of herpes simplex virus type 1.
    J Virol. 1980 Sep;35(3):902-17 PMID: 6252346
  10. Comparative structural analysis of glycoprotein gD of herpes simplex virus types 1 and 2.
    J Virol. 1980 Aug;35(2):428-35 PMID: 6255183
  11. Monoclonal antibodies to two glycoproteins of herpes simplex virus type 2.
    J Virol. 1981 Aug;39(2):438-46 PMID: 6168775
  12. Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies.
    Infect Immun. 1982 Jan;35(1):363-7 PMID: 6172383
  13. Molecular genetics of herpes simplex virus. V. Characterization of a mutant defective in ability to form plaques at low temperatures and in a viral fraction which prevents accumulation of coreless capsids at nuclear pores late in infection.
    J Virol. 1981 Dec;40(3):870-80 PMID: 6275122
  14. Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP 4.
    Infect Immun. 1981 Dec;34(3):684-92 PMID: 6277788
  15. Sulphated glycoproteins induced by herpes simplex virus.
    J Gen Virol. 1982 Feb;58(Pt 2):399-415 PMID: 6278062
  16. Glycoprotein gE of herpes simplex virus type 1: effects of anti-gE on virion infectivity and on virus-induced fc-binding receptors.
    J Virol. 1982 Jan;41(1):129-36 PMID: 6283107
  17. Similarities and differences in the Fc-binding glycoprotein (gE) of herpes simplex virus types 1 and 2 and tentative mapping of the viral gene for this glycoprotein.
    J Virol. 1982 Jan;41(1):137-44 PMID: 6283108
  18. Location of the structural genes for glycoproteins gD and gE and for other polypeptides in the S component of herpes simplex virus type 1 DNA.
    J Virol. 1982 Jul;43(1):41-9 PMID: 6287015
  19. Glycoproteins of herpes simplex virus type 2 as defined by monoclonal antibodies.
    J Virol. 1982 Oct;44(1):344-55 PMID: 6292496
  20. Expression of herpes simplex virus glycoprotein C from a DNA fragment inserted into the thymidine kinase gene of this virus.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6612-6 PMID: 6292909
  21. Detailed analysis of the portion of the herpes simplex virus type 1 genome encoding glycoprotein C.
    J Virol. 1983 Feb;45(2):634-47 PMID: 6300426
  22. Proteins specified by herpes simplex virus. XII. The virion polypeptides of type 1 strains.
    J Virol. 1974 Sep;14(3):640-51 PMID: 4369085
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.45.3.1223-1227.1983
Published
1983-03-00
Pages
1223-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256538
Subset
IM
Grants
NIGMS NIH HHS · 5 T32 GM07197 · United States
NCI NIH HHS · CA 19264 · United States
NCI NIH HHS · CA 21776 · United States
External Links
PubMed source DOI: 10.1128/JVI.45.3.1223-1227.1983 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2