Home LiteratureArticle Details
PMID: 6300769 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene.

Nucleic acids research ·Vol. 11 ·No. 3 ·1983-02-11 ·Pages 837-51

Brooks JE, Blumenthal RM, Gingeras TR

Abstract

The E. coli dam (DNA adenine methylase) enzyme is known to methylate the sequence GATC. A general method for cloning sequence-specific DNA methylase genes was used to isolate the dam gene on a 1.14 kb fragment, inserted in the plasmid vector pBR322. Subsequent restriction mapping and subcloning experiments established a set of approximate boundaries of the gene. The nucleotide sequence of the dam gene was determined, and analysis of that sequence revealed a unique open reading frame which corresponded in length to that necessary to code for a protein the size of dam. Amino acid composition derived from this sequence corresponds closely to the amino acid composition of the purified dam protein. Enzymatic and DNA:DNA hybridization methods were used to investigate the possible presence of dam genes in a variety of prokaryotic organisms.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA (Cytosine-5-)-Methyltransferases/genetics DNA Restriction Enzymes DNA, Bacterial/genetics,isolation & purification Escherichia coli/enzymology Genes Genes, Bacterial Methyltransferases/genetics Nucleic Acid Hybridization Plasmids
Chemicals
DNA, Bacterial Methyltransferases DNA (Cytosine-5-)-Methyltransferases DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brooks J E
Blumenthal R M
Gingeras T R
References (49)
49 references, click to expand
  1. The nucleotide sequence of the structural gene for Escherichia coli tryptophanyl-tRNA synthetase.
    J Biol Chem. 1982 Jun 10;257(11):6132-6 PMID: 7042706
  2. DNA methyltransferase-dependent transcription of the phage Mu mom gene.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5518-21 PMID: 6752951
  3. Primary structure of the chromosomal origins (oriC) of Enterobacter aerogenes and Klebsiella pneumoniae: comparisons and evolutionary relationships.
    J Bacteriol. 1982 Jun;150(3):1467-71 PMID: 7042695
  4. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  5. Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break.
    J Biol Chem. 1970 Jan 10;245(1):39-45 PMID: 4904090
  6. Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli.
    J Bacteriol. 1972 Jun;110(3):1135-46 PMID: 4555406
  7. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  8. Isolation of deoxyribonucleic acid methylase mutants of Escherichia coli K-12.
    J Bacteriol. 1973 Jun;114(3):1143-50 PMID: 4576399
  9. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
    Biochemistry. 1973 Jul 31;12(16):3055-63 PMID: 4354250
  10. Location of DNA methylation genes on the Escherichia coli K-12 genetic map.
    Mol Gen Genet. 1973 Dec 14;127(1):47-55 PMID: 4589344
  11. Biological function for 6-methyladenine residues in the DNA of Escherichia coli K12.
    J Mol Biol. 1974 May 15;85(2):309-22 PMID: 4600143
  12. A deoxyribonuclease of Diplococcus pneumoniae specific for methylated DNA.
    J Biol Chem. 1975 Jun 10;250(11):4060-66 PMID: 236309
  13. Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12.
    Mutat Res. 1975 Apr;28(1):15-26 PMID: 167279
  14. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  15. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.
    Cell. 1976 Sep;9(1):91-9 PMID: 788919
  16. Adenine methylation of Okazaki fragments in Escherichia coli.
    J Bacteriol. 1976 Dec;128(3):853-4 PMID: 791938
  17. Repair tracts in mismatched DNA heteroduplexes.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4135-9 PMID: 1069303
  18. Ends of bacteriophage mu DNA.
    Nature. 1976 Dec 9;264(5586):580-3 PMID: 1004602
  19. A restriction endonuclease from Staphylococcus aureus.
    Nucleic Acids Res. 1976 Nov;3(11):3193-202 PMID: 1005115
  20. Hyper-recombination in dam mutants of Escherichia coli K-12.
    Mol Gen Genet. 1976 Dec 22;149(3):273-7 PMID: 799245
  21. DNA modification of bacteriophage Mu-1 requires both host and bacteriophage functions.
    J Virol. 1977 Sep;23(3):825-6 PMID: 330882
  22. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation.
    J Mol Biol. 1977 Jul;114(1):153-68 PMID: 20509
  23. Two sequence-specific endonucleases from Moraxella bovis.
    J Mol Biol. 1977 Jul;114(1):169-79 PMID: 909085
  24. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  25. Endo R DpnI restriction of Escherichia coli DNA synthesized in vitro. Evidence that the ends of Okazaki pieces are determined by template deoxynucleotide sequence.
    J Mol Biol. 1977 Dec 15;117(3):621-35 PMID: 24749
  26. Induced mutagenesis in dam- mutants of Escherichia coli: a role for 6-methyladenine residues in mutation avoidance.
    Mol Gen Genet. 1978 Jul 25;163(3):307-12 PMID: 355857
  27. Nucleotide sequence of Escherichia coli K-12 replication origin.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):575-9 PMID: 370832
  28. Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):580-4 PMID: 370833
  29. The effect of differential methylation by Escherichia coli of plasmid DNA and phage T7 and lambda DNA on the cleavage by restriction endonuclease MboI from Moraxella bovis.
    Biochim Biophys Acta. 1979 May 24;562(3):418-28 PMID: 378259
  30. A new specific endonuclease from Anabaena variabilis.
    FEBS Lett. 1979 Aug 1;104(1):39-44 PMID: 225204
  31. A semi-automated method for the reading of nucleic acid sequencing gels.
    Nucleic Acids Res. 1982 Jan 11;10(1):103-14 PMID: 7063397
  32. SEQ: a nucleotide sequence analysis and recombination system.
    Nucleic Acids Res. 1982 Jan 11;10(1):279-94 PMID: 7063402
  33. Computer programs for nucleic acid sequence manipulation.
    Nucleic Acids Res. 1982 Jan 11;10(1):91-101 PMID: 6278414
  34. Modification profiles of bacterial genomes.
    Nucleic Acids Res. 1982 Feb 11;10(3):913-34 PMID: 6278441
  35. A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis.
    Plasmid. 1980 Jan;3(1):88-91 PMID: 6278527
  36. Computer programs for the assembly of DNA sequences.
    Nucleic Acids Res. 1979 Sep 25;7(2):529-45 PMID: 493154
  37. Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):1063-7 PMID: 6987663
  38. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  39. 5'-32P labeling of RNA and DNA restriction fragments.
    Methods Enzymol. 1980;65(1):75-85 PMID: 6154877
  40. Association of Mu-containing plasmids with the Escherichia coli chromosome upon prophage induction.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1778-82 PMID: 6246503
  41. The nucleotide sequences recognized by endonucleases AvaI and AvaII from Anabaena variabilis.
    Biochem J. 1980 Jan 1;185(1):65-75 PMID: 6246881
  42. Nucleotide sequence of the Salmonella typhimurium origin of DNA replication.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2460-4 PMID: 6248850
  43. DNA methylation and gene function.
    Science. 1980 Nov 7;210(4470):604-10 PMID: 6254144
  44. Nucleotide sequences of heat shock activated genes in Drosophila melanogaster. I. Sequences in the regions of the 5' and 3' ends of the hsp 70 gene in the hybrid plasmid 56H8.
    Nucleic Acids Res. 1980 Jul 25;8(14):3105-23 PMID: 6255408
  45. Escherichia coli K-12 clones that overproduce dam methylase are hypermutable.
    J Bacteriol. 1981 Jan;145(1):644-6 PMID: 7007328
  46. Restriction and modification enzymes and their recognition sequences.
    Nucleic Acids Res. 1981 Jan 10;9(1):r75-96 PMID: 6259615
  47. 5-Methylcytosine in eukaryotic DNA.
    Science. 1981 Jun 19;212(4501):1350-7 PMID: 6262918
  48. Cloning and characterization of the gene for Escherichia coli tryptophanyl-transfer ribonucleic acid synthetase.
    J Bacteriol. 1981 Dec;148(3):941-9 PMID: 6171561
  49. Escherichia coli dam methylase. Physical and catalytic properties of the homogeneous enzyme.
    J Biol Chem. 1982 Mar 10;257(5):2605-12 PMID: 7037767
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.3.837
Published
1983-02-11
Pages
837-51
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325756
Subset
IM
Grants
NCI NIH HHS · 5-R01-CA27275-02 · United States
NCI NIH HHS · CA 09311-02 · United States
Databases
GENBANK
J01600
External Links
PubMed source DOI: 10.1093/nar/11.3.837 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2