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PMID: 6300772 Published · ppublish English Journal Article

The nucleotide sequence of the yeast PHO5 gene: a putative precursor of repressible acid phosphatase contains a signal peptide.

Nucleic acids research ·Vol. 11 ·No. 6 ·1983-03-25 ·Pages 1657-72

Arima K, Oshima T, Kubota I, Nakamura N, Mizunaga T, Toh-e A

Abstract

The nucleotide sequence of the PHO5 gene of the yeast, Saccharomyces cerevisiae, which encodes repressible acid phosphatase (APase) was determined. Comparison of N-terminal amino acid sequence deduced from the nucleotide sequence with that of the purified repressible APase revealed the existence of a putative signal peptide in the precursor protein. The signal peptide was shown to contain 17 amino acid residues and its structural features were quite similar to those of higher eukaryotic and prokaryotic signal peptides. The nucleotide sequence of 5' and 3' noncoding flanking regions of the PHO5 gene are also discussed.

MeSH Terms
Acid Phosphatase/genetics Amino Acid Sequence Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Fungal/genetics Enzyme Repression Escherichia coli/genetics Genes Plasmids Saccharomyces cerevisiae/enzymology,genetics
Chemicals
DNA, Fungal DNA Restriction Enzymes Acid Phosphatase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Arima K
Oshima T
Kubota I
Nakamura N
Mizunaga T
Toh-e A
References (39)
39 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Human erythrocyte membrane glycoprotein: a re-evaluation of the molecular weight as determined by SDS polyacrylamide gel electrophoresis.
    Biochem Biophys Res Commun. 1971 Jul 16;44(2):390-5 PMID: 4334138
  3. Isolation and characterization of acid phosphatase mutants in Saccharomyces cerevisiae.
    J Bacteriol. 1973 Feb;113(2):727-38 PMID: 4570606
  4. Three forms of the 5.8-S ribosomal RNA species in Saccharomyces cerevisiae.
    Eur J Biochem. 1974 Jan 3;41(1):197-202 PMID: 4593336
  5. Biosynthesis of acid phosphatase of baker's yeast. Characterization of a protoplast-bound fraction containing precursors of the exo-enzyme.
    Biochim Biophys Acta. 1975 Feb 19;377(2):331-42 PMID: 235303
  6. Isolation and characterization of recessive, constitutive mutations for repressible acid phosphatase synthesis in Saccharomyces cerevisiae.
    J Bacteriol. 1975 Jun;122(3):911-22 PMID: 1097406
  7. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
    J Cell Biol. 1975 Dec;67(3):835-51 PMID: 811671
  8. Genes coding for the structure of the acid phosphatases in Saccharomyces cerevisiae.
    Mol Gen Genet. 1975 Dec 30;143(1):65-70 PMID: 765744
  9. Gene duplication in Saccharomyces cerevisiae.
    Genetics. 1978 Apr;88(4 Pt 1):673-87 PMID: 348562
  10. Functional expression of cloned yeast DNA in Escherichia coli: specific complementation of argininosuccinate lyase (argH) mutations.
    J Mol Biol. 1978 Apr 25;120(4):517-32 PMID: 349166
  11. Transformation of yeast by a replicating hybrid plasmid.
    Nature. 1978 Sep 14;275(5676):104-9 PMID: 357984
  12. Isolation and characterisation of a yeast chromosomal replicator.
    Nature. 1979 Nov 1;282(5734):39-43 PMID: 388229
  13. The mechanism of protein secretion across membranes.
    Nature. 1980 Jan 31;283(5746):433-8 PMID: 7352023
  14. A stable plasmid carrying the yeast Leu2 gene and containing only yeast deoxyribonucleic acid.
    J Bacteriol. 1980 Jan;141(1):413-6 PMID: 6243628
  15. Structural comparison of two nontandemly repeated yeast glyceraldehyde-3-phosphate dehydrogenase genes.
    J Biol Chem. 1980 Mar 25;255(6):2596-605 PMID: 6244283
  16. Isolation and sequence of the gene for iso-2-cytochrome c in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):541-5 PMID: 6244566
  17. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  18. Mutations which alter the function of the signal sequence of the maltose binding protein of Escherichia coli.
    Nature. 1980 May 8;285(5760):78-81 PMID: 6990274
  19. Secretion and membrane localization of proteins in Escherichia coli.
    CRC Crit Rev Biochem. 1980;7(4):339-71 PMID: 6993100
  20. Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.
    Gene. 1980 Jul;10(2):157-66 PMID: 6248420
  21. Eukaryotic signal sequence transports insulin antigen in Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3369-73 PMID: 6251458
  22. Promoter sequences of eukaryotic protein-coding genes.
    Science. 1980 Sep 19;209(4463):1406-14 PMID: 6251548
  23. In vitro synthesis of repressible yeast acid phosphatase: identification of multiple mRNAs and products.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4504-8 PMID: 7001459
  24. The primary structures of two yeast enolase genes. Homology between the 5' noncoding flanking regions of yeast enolase and glyceraldehyde-3-phosphate dehydrogenase genes.
    J Biol Chem. 1981 Feb 10;256(3):1385-95 PMID: 6256394
  25. Isolation of yeast genes with mRNA levels controlled by phosphate concentration.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6541-5 PMID: 6256743
  26. Structure and function of the PHO82-pho4 locus controlling the synthesis of repressible acid phosphatase of Saccharomyces cerevisiae.
    J Bacteriol. 1981 Jan;145(1):221-32 PMID: 7007314
  27. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  28. N-Glycosylation of yeast proteins. Characterization of the solubilized oligosaccharyl transferase.
    Eur J Biochem. 1981 May;116(1):101-8 PMID: 7018901
  29. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.
    Nucleic Acids Res. 1981 Oct 24;9(20):5233-52 PMID: 7301588
  30. Codon selection in yeast.
    J Biol Chem. 1982 Mar 25;257(6):3026-31 PMID: 7037777
  31. Presecretory and cytoplasmic invertase polypeptides encoded by distinct mRNAs derived from the same structural gene differ by a signal sequence.
    Proc Natl Acad Sci U S A. 1982 Feb;79(3):781-5 PMID: 7038684
  32. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
    Cell. 1982 Jan;28(1):145-54 PMID: 7039847
  33. The role of core-oligosaccharide in formation of an active acid phosphatase and its secretion by yeast protoplasts.
    J Biochem. 1982 Jan;91(1):191-200 PMID: 6802810
  34. Expression in Escherichia coli of chemically synthesized gene for a novel opiate peptide alpha-neo-endorphin.
    Nucleic Acids Res. 1982 Mar 11;10(5):1741-54 PMID: 6280156
  35. DNA sequence required for efficient transcription termination in yeast.
    Cell. 1982 Mar;28(3):563-73 PMID: 6280875
  36. Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1982 Apr 24;10(8):2625-37 PMID: 6281737
  37. Cloning of the HIS5 gene of Saccharomyces cerevisiae by yeast transformation.
    Gene. 1981 Dec;16(1-3):335-41 PMID: 7044894
  38. Acid phosphatase polypeptides in Saccharomyces cerevisiae are encoded by a differentially regulated multigene family.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2157-61 PMID: 6212932
  39. Two yeast acid phosphatase structural genes are the result of a tandem duplication and show different degrees of homology in their promoter and coding sequences.
    EMBO J. 1982;1(6):675-80 PMID: 6329697
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.6.1657
Published
1983-03-25
Pages
1657-72
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325827
Subset
IM
Databases
GENBANK
J01343
External Links
PubMed source DOI: 10.1093/nar/11.6.1657 PMC Full Text View journal details
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