Home LiteratureArticle Details

PMID: 6300832 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Detection and partial characterization of a chymostatin-sensitive endopeptidase in transformed fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 2 ·1983-01-00 ·Pages 344-8

O'Donnell-Tormey J, Quigley JP

Abstract

A chymostatin-sensitive step in the release of plasminogen activator from transformed fibroblasts has been described recently. By using synthetic peptidyl substrates, we have detected and characterized a chymostatin-sensitive peptidase activity in chicken embryo fibroblasts transformed by Rous sarcoma virus. The activity represents a neutral endopeptidase that exhibits phenylalanine specificity and is inhibited by diisopropyl fluorophosphate. A detailed inhibitor profile of the enzyme activity shows that it is distinct from other chymotrypsin-like phenylalanine-preferring peptidases. The endopeptidase activity in transformed fibroblasts is increased over that of parallel cultures of normal fibroblasts. The mechanism of enzyme inhibition by chymostatin is indicated by these studies, and the possible role of the enzyme in modulating plasminogen activator secretion is discussed.

MeSH Terms

Amino Acid Sequence Animals Avian Sarcoma Viruses/genetics Cell Transformation, Neoplastic Chick Embryo Endopeptidases/isolation & purification,metabolism Fibroblasts/enzymology Kinetics Oligopeptides/pharmacology Protease Inhibitors/pharmacology Substrate Specificity

Chemicals

Oligopeptides Protease Inhibitors chymostatin Endopeptidases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

O'Donnell-Tormey J

Quigley J P

References (23)

23 references, click to expand

Specificity of porcine pancreatic elastase, human leukocyte elastase and cathepsin G. Inhibition with peptide chloromethyl ketones.
Biochim Biophys Acta. 1977 Nov 23;485(1):156-66 PMID: 562189
Purification of plasminogen activator from Rous sarcoma virus transformed chick embryo fibroblasts treated with the tumor promoter phorbol 12-myristate 13-acetate.
Biochemistry. 1980 Nov 25;19(24):5463-71 PMID: 6257279
Cationic proteins from human neutrophil granulocytes. Evidence for their chymotrypsin-like properties.
Biochim Biophys Acta. 1975 Feb 27;379(2):606-17 PMID: 235318
A zinc metalloendopeptidase associated with dog pancreatic membranes.
J Biol Chem. 1980 Mar 25;255(6):2227-30 PMID: 6987219
Human cathepsin G. Catalytic and immunological properties.
Biochem J. 1976 May 1;155(2):273-8 PMID: 7245
Direct fluorescent assay of urokinase and plasminogen activators of normal and malignant cells: kinetics and inhibitor profiles.
Proc Natl Acad Sci U S A. 1978 Feb;75(2):750-3 PMID: 204931
Shedding from the cell surface of normal and cancer cells.
Adv Cancer Res. 1980;32:75-199 PMID: 7008543
Inhibition of collagen secretion from bone and cultured fibroblasts by microtubular disruptive drugs.
Proc Natl Acad Sci U S A. 1972 Apr;69(4):892-6 PMID: 4502941
Sbustrate specificity of the elastase and the chymotrypsin-like enzyme of the human granulocyte.
Biochim Biophys Acta. 1977 Jan 11;480(1):241-5 PMID: 401649
New hypothesis of insulin secretion.
Nature. 1968 Sep 14;219(5159):1177-9 PMID: 4877537
Partial purification and characterization of a chymotrypsin-like enzyme from human neutrophil leucocytes.
Biochim Biophys Acta. 1974 Sep 11;364(1):103-12 PMID: 4433560
Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
J Cell Biol. 1975 Dec;67(3):835-51 PMID: 811671
Post-translational cleavage of presecretory proteins with an extract of rough microsomes from dog pancreas containing signal peptidase activity.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5598-602 PMID: 271987
Time lag in the secretion of collagen by matrix-free tendon cells and inhibition of the secretory process by colchicine and vinblastine.
Biochim Biophys Acta. 1972 Apr 21;264(2):375-82 PMID: 4337621
A rapid method for purification of human granulocyte cationic neutral proteases: purification and characterization of human granulocyte chymotrypsin-like enzyme.
Biochim Biophys Acta. 1975 Oct 22;403(2):477-92 PMID: 1081003
Association of a protease (plasminogen activator) with a specific membrane fraction isolated from transformed cells.
J Cell Biol. 1976 Nov;71(2):472-86 PMID: 993259
Purification and properties of a mast cell protease.
J Biol Chem. 1966 Nov 10;241(21):5090-4 PMID: 5925871
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Possible role of microtubules in thyroid secretion.
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1901-8 PMID: 4321351
Inhibition of plasminogen activator release from transformed chicken fibroblasts by a protease inhibitor.
Cell. 1981 Nov;27(1 Pt 2):85-95 PMID: 6276025
The effect of pH on the affinities of enzymes for substrates and inhibitors.
Biochem J. 1953 Aug;55(1):161-70 PMID: 13093634
Preparative purification of the rat mast cell chymase: characterization and interaction with granule components.
J Exp Med. 1977 Nov 1;146(5):1405-19 PMID: 336834
Human alpha-1-antichymotrypsin: interaction with chymotrypsin-like proteinases.
Biochemistry. 1978 Dec 26;17(26):5651-6 PMID: 728423

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.80.2.344

Published

1983-01-00

Pages

344-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC393373

Subset

Grants

NCI NIH HHS · CA26740 · United States

External Links

PubMed source DOI: 10.1073/pnas.80.2.344 PMC Full Text View journal details

Analysis Services

Detection and partial characterization of a chymostatin-sensitive endopeptidase in transformed fibroblasts.

Contact

WeChat Official Account