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PMID: 6302690 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

recF-dependent induction of recA synthesis by coumermycin, a specific inhibitor of the B subunit of DNA gyrase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 9 ·1983-05-00 ·Pages 2510-3

Smith CL

Abstract

Genetic and biochemical studies on enzymes known to be involved in regulating DNA supercoiling yield a complex spectrum of effects on the Escherichia coli SOS system. Previous studies indicated that only inhibition of DNA gyrase by antibiotics that act on the DNA gyrase A subunit results in turning on the E. coli SOS system. Here we show that coumermycin, an antibiotic that acts on the DNA gyrase B subunit, can also induce. Like nalidixic acid induction, coumermycin induction is dependent on the recBC DNase. In both cases induction apparently results from a response of the cell to the DNA gyrase-inhibitor complex rather than just the loss of DNA gyrase activity. However, unlike induction by the DNA gyrase A-specific antibiotics, coumermycin induction also requires the recF gene product. This demonstrates a functional relationship between DNA gyrase and the recF gene product.

MeSH Terms
Aminocoumarins Bacterial Proteins/biosynthesis,metabolism Coumarins/pharmacology DNA Replication/drug effects DNA, Superhelical/biosynthesis Deoxyribonucleases/metabolism Enzyme Induction Escherichia coli Escherichia coli Proteins Exodeoxyribonuclease V Macromolecular Substances Rec A Recombinases Topoisomerase II Inhibitors
Chemicals
Aminocoumarins Bacterial Proteins Coumarins DNA, Superhelical Escherichia coli Proteins Macromolecular Substances Topoisomerase II Inhibitors Rec A Recombinases Deoxyribonucleases Exodeoxyribonuclease V exodeoxyribonuclease V, E coli coumermycin
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Smith C L
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.9.2510
Published
1983-05-00
Pages
2510-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393855
Subset
IM
Grants
NIGMS NIH HHS · GM26103 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.9.2510 PMC Full Text View journal details
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