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PMID: 6304107 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Reversible control of synaptic transmission in a single gene mutant of Drosophila melanogaster.

The Journal of cell biology ·Vol. 96 ·No. 6 ·1983-06-00 ·Pages 1517-22

Koenig JH, Saito K, Ikeda K

Abstract

Synaptic transmission of the single gene mutant, shibirets1 (shi), of Drosophila melanogaster is reversibly blocked by elevated temperature. The presynaptic mechanism of transmission was studied in the neuromuscular junction of the dorsal longitudinal flight muscle of this mutant. It was observed that when the temperature was raised to 29 degrees C in shi flies, the amplitude of the excitatory junction potential (EJP) greatly diminished, the frequency of spontaneously released miniature excitatory junction potentials (MEJP's) was greatly reduced, and almost complete vesicle depletion was observed. These conditions were reversible if the temperature was lowered to 19 degrees C. These data suggest that the block in transmission is a result of vesicle depletion. It is suggested that depletion occurs not as a result of excessive release of transmitter but rather as a result of a block in the recycling of vesicles, which causes depletion as exocytosis (transmitter release) proceeds normally.

MeSH Terms
Animals Drosophila melanogaster/genetics,physiology Evoked Potentials Mutation Neuromuscular Junction/physiology,ultrastructure Synapses/physiology Synaptic Transmission Temperature
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Koenig J H
Saito K
Ikeda K
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20 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.96.6.1517
Published
1983-06-00
Pages
1517-22
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112451
Subset
IM
Grants
NINDS NIH HHS · NS-18856 · United States
External Links
PubMed source DOI: 10.1083/jcb.96.6.1517 PMC Full Text View journal details
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