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PMID: 6304110 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A study on sensing and adaptation in Dictyostelium discoideum: guanosine 3',5'-phosphate accumulation and light-scattering responses.

The Journal of cell biology ·Vol. 96 ·No. 6 ·1983-06-00 ·Pages 1566-70

Wurster B, Butz U

Abstract

Cells of Dictyostelium discoideum respond to extracellular cyclic AMP with marked changes in intracellular cyclic GMP levels and light scattering. In this work, defined temporal increases in cyclic AMP were produced by the continuous addition of cyclic AMP to agitated suspensions of cells; concomitant hydrolysis of cyclic AMP by the cells subsequently established a constant, steady state concentration. The cells responded to the initial increase in extracellular cyclic AMP with a rapid increase in the intracellular cyclic GMP concentration and a rapid decrease in light scattering. At cyclic AMP input rates of 0.5-5 nM X s-1, the fast reactions of cyclic GMP and light scattering had already relaxed while the cyclic AMP concentration in the cell suspension was still increasing. The cells responded to constant concentrations of cyclic AMP with constant elevated cyclic GMP concentrations and constant decreased levels of light scattering. Our results are consistent with the existence of two types of perception systems, one of which adapts to constant stimuli and one of which does not adapt.

MeSH Terms
Chemotaxis/drug effects Cyclic AMP/pharmacology Cyclic GMP/metabolism Dictyostelium/cytology,metabolism Light Mathematics Scattering, Radiation
Chemicals
Cyclic AMP Cyclic GMP
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wurster B
Butz U
References (48)
48 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.96.6.1566
Published
1983-06-00
Pages
1566-70
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112470
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.96.6.1566 PMC Full Text View journal details
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