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PMID: 6304462 Published · ppublish English Journal Article

Expression of a neomycin phosphotransferase gene from Streptomyces fradiae in Escherichia coli after interplasmidic recombination.

Molecular & general genetics : MGG ·Vol. 189 ·No. 1 ·1983-00-00 ·Pages 27-33

Schupp T, Toupet C, Stålhammar-Carlemalm M, Meyer J

Abstract

Plasmid pIJ2 carrying the neomycin phosphotransferase gene of Streptomyces fradiae was fused to E. coli plasmid pBR325 and the hybrid molecules were introduced into E. coli K12 by transformation. The neomycin phosphotransferase gene of the hybrid plasmid was not expressed in E. coli, except after interplasmidic recombination. Physical analysis of such an in vivo recombinant plasmid revealed that the recombination brought one neomycin phosphotransferase gene to a position downstream from the tet-promoter of pBR325. Subcloning experiments indicated that this is the gene copy expressed, and that transcription is initiated at the tet-promoter of pBR325.

MeSH Terms
Escherichia coli/genetics Gene Expression Regulation Genes Genes, Bacterial Kanamycin Kinase Operon Phosphotransferases/genetics Plasmids Recombination, Genetic Streptomyces/genetics
Chemicals
Phosphotransferases Kanamycin Kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schupp T
Toupet C
Stålhammar-Carlemalm M
Meyer J
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30 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00326051
Published
1983-00-00
Pages
27-33
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00326051
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