Expression of human immunoglobulin E epsilon chain cDNA in E. coli.

Nucleic acids research ·Vol. 11 ·No. 10 ·1983-05-25 ·Pages 3077-85

Kurokawa T, Seno M, Sasada R, Ono Y, Onda H, Igarashi K, Kikuchi M, Sugino Y, Honjo T

Abstract

Using the cDNA of human epsilon chain, three expression plasmids that code directly the constant portion of the epsilon chain (C epsilon 1-C epsilon 4, C epsilon 2-C epsilon 4 and C epsilon 3-C epsilon 4 domains) were constructed. These epsilon chain peptides were synthesized in E. coli under the control of the trp promoter-operator. The bacterially produced peptides have the antigenicity of human epsilon chain and gave the molecular weights equal to the values calculated from the amino acid sequence of the constructed plasmids.

MeSH Terms

Base Sequence Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Escherichia coli/genetics Humans Immunoglobulin E/genetics Immunoglobulin Heavy Chains/genetics Immunoglobulin epsilon-Chains/genetics Operon Plasmids

Chemicals

Immunoglobulin Heavy Chains Immunoglobulin epsilon-Chains Immunoglobulin E DNA DNA Restriction Enzymes

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Kurokawa T

Seno M

Sasada R

Ono Y

Onda H

Igarashi K

Kikuchi M

Sugino Y

Honjo T

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/11.10.3077

Published

1983-05-25

Pages

3077-85

Language

English

Region

England

NLM ID

0411011

PMCID

PMC325949

Subset

External Links

PubMed source DOI: 10.1093/nar/11.10.3077 PMC Full Text View journal details

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Expression of human immunoglobulin E epsilon chain cDNA in E. coli.

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