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PMID: 6304622 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structural perturbation in supercoiled DNA: hypersensitivity to modification by a single-strand-selective chemical reagent conferred by inverted repeat sequences.

Nucleic acids research ·Vol. 11 ·No. 10 ·1983-05-25 ·Pages 3097-112

Lilley DM

Abstract

Bromoacetaldehyde, a reagent which modifies unpaired adenine residues, selectively modifies supercoiled DNA in the region of inverted repeats which are known targets for single-strand-specific nucleases. The reaction is dependent upon the topological state of the molecule, and the absolute importance of the inverted repeat has been demonstrated. Finer mapping of the distribution of the modification pattern reveals significant and interesting differences from the S1 nuclease target positions. Bromoacetaldehyde modification is distributed over a wider region covering the whole inverted repeat, with greatest extent of reaction in the regions which flank the inverted repeat. It is suggested that an altered conformation may be propagated into these sequences. These results further support the contention that inverted repeats adopt an altered conformation when negatively supercoiled, for which the principal suggestion remains the cruciform structure.

MeSH Terms
Acetaldehyde/analogs & derivatives DNA Restriction Enzymes DNA, Single-Stranded DNA, Superhelical Nucleic Acid Conformation Phosphorus Radioisotopes Plasmids Polynucleotide 5'-Hydroxyl-Kinase/metabolism Repetitive Sequences, Nucleic Acid T-Phages/enzymology
Chemicals
DNA, Single-Stranded DNA, Superhelical Phosphorus Radioisotopes bromoacetaldehyde Polynucleotide 5'-Hydroxyl-Kinase DNA Restriction Enzymes Acetaldehyde
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Lilley D M
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.10.3097
Published
1983-05-25
Pages
3097-112
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325951
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.10.3097 PMC Full Text View journal details
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