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PMID: 6304630 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structure of mouse rRNA precursors. Complete sequence and potential folding of the spacer regions between 18S and 28S rRNA.

Nucleic acids research ·Vol. 11 ·No. 10 ·1983-05-25 ·Pages 3375-91

Michot B, Bachellerie JP, Raynal F

Abstract

We have determined the complete nucleotide sequence of the regions of mouse ribosomal RNA transcription unit which separate mature rRNA genes. These internal transcribed spacers (ITS) are excised from rRNA precursor during ribosome biosynthesis. ITS 1, between 18S and 5.8S rRNA genes, is 999 nucleotides long. ITS 2, between 5.8S and 28S rRNA genes, is 1089 nucleotides long. Both spacers are very rich in G + C, 70 and 74% respectively. Mouse sequences have been compared with the other available eukaryotes: while no homology is apparent with yeast or xenopus, mouse and rat ITS sequences have been largely conserved, with homologous segments interspersed with highly divergent tracts. Homology with rat is much more extensive for ITS 1 than for ITS 2. Tentative secondary structure models are proposed for the folding of these regions within rRNA precursor; they are closely related in mouse and rat.

MeSH Terms
Animals Base Sequence DNA/genetics DNA Restriction Enzymes DNA, Ribosomal Genes Mice Molecular Weight Nucleic Acid Conformation RNA, Ribosomal/genetics Rats Ribosomes/metabolism Saccharomyces cerevisiae/genetics Species Specificity Transcription, Genetic Xenopus
Chemicals
DNA, Ribosomal RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Michot B
Bachellerie J P
Raynal F
References (42)
42 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.10.3375
Published
1983-05-25
Pages
3375-91
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325970
Subset
IM
Databases
GENBANK
J00346, J00623, J00624, V00851
External Links
PubMed source DOI: 10.1093/nar/11.10.3375 PMC Full Text View journal details
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