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PMID: 6304633 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complex endonucleolytic cleavage pattern during early events in the processing of pre-rRNA in the lower eukaryote, Tetrahymena thermophila.

Nucleic acids research ·Vol. 11 ·No. 11 ·1983-06-11 ·Pages 3487-502

Kister KP, Müller B, Eckert WA

Abstract

We have analysed nuclear RNA from T. thermophila by RNA transfer hybridization using cloned rDNA fragments. A very high number of in vivo intermediates and by-products of rRNA processing were identified. These include putative intermediates of the splicing process and alternative products resulting from temporal variability in various endonucleolytic cleavages. In addition, four small RNA species including only transcribed spacer sequences were detected. These are (1) the IVS RNA (approximately 400 bases), the by-product of the splicing process, (2) a fragment from the internal transcribed spacer (approximately 360 bases), possibly resulting from 3'-end processing of pre-17S rRNA, (3) a fragment comprising most or all of the external transcribed spacer (approximately 600 bases) obviously representing the major by-product of 5'-end processing, and, in addition, (4) a small fragment from the initiation region (approximately 230 bases) which might be a product of premature transcription termination.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA/genetics DNA Restriction Enzymes/metabolism DNA, Ribosomal Kinetics Nucleic Acid Hybridization Nucleic Acid Precursors/genetics Plasmids RNA Precursors RNA, Ribosomal/genetics Tetrahymena/genetics Transcription, Genetic
Chemicals
DNA, Ribosomal Nucleic Acid Precursors RNA Precursors RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kister K P
Müller B
Eckert W A
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.11.3487
Published
1983-06-11
Pages
3487-502
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325981
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.11.3487 PMC Full Text View journal details
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