Home LiteratureArticle Details

PMID: 6304643 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

BAL 31 nuclease as a probe in concentrated salt for the B-Z DNA junction.

Nucleic acids research ·Vol. 11 ·No. 11 ·1983-06-11 ·Pages 3811-22

Kilpatrick MW, Wei CF, Gray HB, Wells RD

Abstract

The BAL 31 nuclease, an extracellular nuclease from A. espejiana, specifically recognizes and cleaves the salt induced conformational junction between B and Z-DNA. Short segments of (dC-dG) left-handed Z-helix, comprising approximately 1% of the total DNA, are specifically detected within two different recombinant plasmids. The BAL 31 enzyme is highly resistant to inactivation by the presence of high concentrations of a variety of electrolytes that stabilize left-handed helices, is active at physiological pH, and can be used to probe both linear and circular DNAs. Additionally, the nuclease cleaves left-handed (dC-dG)n only very poorly, if at all. Thus, the BAL 31 nuclease can be utilized as a probe for helical junctions and consequently for segments of left-handed DNA that might exist within predominantly right-handed naturally occurring genomes.

MeSH Terms

Bacteria/enzymology Base Sequence DNA DNA Restriction Enzymes Deoxyribonuclease EcoRI Endodeoxyribonucleases/metabolism Kinetics Nucleic Acid Conformation Osmolar Concentration Plasmids Sodium Chloride/pharmacology Substrate Specificity

Chemicals

Sodium Chloride DNA Endodeoxyribonucleases exonuclease Bal 31 DNA Restriction Enzymes Deoxyribonuclease EcoRI

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Kilpatrick M W

Wei C F

Gray H B

Wells R D

References (20)

20 references, click to expand

Physicochemical studies on polydeoxyribonucleotides containing defined repeating nucleotide sequences.
J Mol Biol. 1970 Dec 28;54(3):465-97 PMID: 5492018
Extracellular nucleases of Pseudomonas BAL 31. I. Characterization of single strand-specific deoxyriboendonuclease and double-strand deoxyriboexonuclease activities.
Nucleic Acids Res. 1975 Sep;2(9):1459-92 PMID: 1178526
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
A sensitive endonuclease probe for lesions in deoxyribonucleic acid helix structure produced by carcinogenic or mutagenic agents.
J Biol Chem. 1977 Dec 10;252(23):8740-6 PMID: 925019
Extracellular nucleases of Alteromonas espejiana BAL 31.IV. The single strand-specific deoxyriboendonuclease activity as a probe for regions of altered secondary structure in negatively and positively supercoiled closed circular DNA.
Nucleic Acids Res. 1979 Jan;6(1):331-57 PMID: 424296
Construction and mapping of recombinant plasmids used for the preparation of DNA fragments containing the Escherichia coli lactose operator and promoter.
J Biol Chem. 1979 Jun 25;254(12):5527-34 PMID: 376510
DNA structure and gene regulation.
Prog Nucleic Acid Res Mol Biol. 1980;24:167-267 PMID: 7005967
Left-handed DNA in restriction fragments and a recombinant plasmid.
Nature. 1981 Apr 23;290(5808):672-7 PMID: 6261149
Conditions which cause the right-handed to left-handed DNA conformational transitions. Evidence for several types of left-handed DNA structures in solution.
J Biol Chem. 1982 Mar 25;257(6):2775-82 PMID: 6277914
Conformation and dynamics in a Z-DNA tetramer.
J Mol Biol. 1981 Nov 15;152(4):723-36 PMID: 7334519
Relationship between superhelical density and cruciform formation in plasmid pVH51.
J Biol Chem. 1982 Jun 10;257(11):6292-5 PMID: 6281266
Junction between Z and B conformations in a DNA restriction fragment: evaluation by Raman spectroscopy.
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2549-53 PMID: 7045865
Left-handed DNA. Cloning, characterization, and instability of inserts containing different lengths of (dC-dG) in Escherichia coli.
J Biol Chem. 1982 Sep 10;257(17):10152-8 PMID: 6286641
Energetic and structural inter-relationship between DNA supercoiling and the right- to left-handed Z helix transitions in recombinant plasmids.
J Biol Chem. 1982 Sep 10;257(17):10159-65 PMID: 6286642
Spectroscopic studies on acetylaminofluorene-modified (dT-dG)n . (dC-dA)n suggest a left-handed conformation.
J Biol Chem. 1982 Sep 10;257(17):10166-71 PMID: 6213617
Left-handed Z-DNA is induced by supercoiling in physiological ionic conditions.
Nature. 1982 Sep 23;299(5881):312-6 PMID: 6287292
The three-dimensional structure of DNA.
Annu Rev Biochem. 1982;51:395-427 PMID: 7051961
An altered DNA conformation detected by S1 nuclease occurs at specific regions in active chick globin chromatin.
Cell. 1982 Jun;29(2):609-22 PMID: 6288265
Flipping of cloned d(pCpG)n.d(pCpG)n DNA sequences from right- to left-handed helical structure by salt, Co(III), or negative supercoiling.
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4560-4 PMID: 6956879
Z* DNA, the left-handed helical form of poly[d(G-C)] in MgCl2-ethanol, is biologically active.
EMBO J. 1982;1(1):115-20 PMID: 6232131

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/11.11.3811

Published

1983-06-11

Pages

3811-22

Language

English

Region

England

NLM ID

0411011

PMCID

PMC326004

Subset

Grants

NCI NIH HHS · CA-11761 · United States

NCI NIH HHS · CA-20279 · United States

NIGMS NIH HHS · GM-21839 · United States

External Links

PubMed source DOI: 10.1093/nar/11.11.3811 PMC Full Text View journal details

Analysis Services

BAL 31 nuclease as a probe in concentrated salt for the B-Z DNA junction.

Contact

WeChat Official Account