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PMID: 6304645 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The human thyroglobulin gene contains two 15-17 kb introns near its 3'-end.

Nucleic acids research ·Vol. 11 ·No. 8 ·1983-04-25 ·Pages 2273-85

van Ommen GJ, Arnberg AC, Baas F, Brocas H, Sterk A, Tegelaers WH, Vassart G, de Vijlder JJ

Abstract

We have cloned overlapping segments of the human thyroglobulin gene from a genomic cosmid library. Restriction mapping and electron microscopy show that a region of 38 kb at or near the 3'-end of this gene encodes only 850 nucleotides or 10% of the messenger RNA (mRNA) sequence. The region contains five exons of 130-210 nucleotides, split by introns of 1 to 15-17 kb. This represents the lowest ratio of coding to non-coding DNA (2.2%) found thus far in any eukaryotic gene. Blot hybridization under non-stringent conditions shows the presence of only one copy of this gene in the human genome and the absence of other closely related sequences.

MeSH Terms
Base Composition Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes Genetic Code Humans Microscopy, Electron Nucleic Acid Conformation Plasmids RNA, Messenger/genetics Thyroglobulin/genetics
Chemicals
RNA, Messenger Thyroglobulin DNA Restriction Enzymes
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
van Ommen G J
Arnberg A C
Baas F
Brocas H
Sterk A
Tegelaers W H
Vassart G
de Vijlder J J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.8.2273
Published
1983-04-25
Pages
2273-85
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325883
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.8.2273 PMC Full Text View journal details
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