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PMID: 6304701 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning of the gene for C protein, a low molecular weight spore-specific protein from Bacillus megaterium.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 11 ·1983-06-00 ·Pages 3250-4

Curiel-Quesada E, Setlow B, Setlow P

Abstract

The structural gene for C protein, a low molecular weight spore-specific protein from Bacillus megaterium, has been cloned in Escherichia coli. Expression of the C-protein gene in E. coli requires an external transcription promoter and prevention of termination of transcription prior to transcription of all or part of the sequence coding for the C protein. The gene for the C protein is within a 5-kilobase DNA fragment, but this fragment does not code for either of the other two major low molecular weight spore proteins, suggesting that the structural genes for these proteins are not tightly linked.

MeSH Terms
Bacillus megaterium/genetics,metabolism Bacterial Proteins/genetics Base Sequence Cloning, Molecular DNA Replication DNA Restriction Enzymes Escherichia coli/genetics Genes Molecular Weight Plasmids Spores, Bacterial/metabolism
Chemicals
Bacterial Proteins DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Curiel-Quesada E
Setlow B
Setlow P
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.11.3250
Published
1983-06-00
Pages
3250-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC394018
Subset
IM
Grants
NIGMS NIH HHS · GM 19698 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.11.3250 PMC Full Text View journal details
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