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PMID: 6304735 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection of HLA-D/DR-related DNA polymorphism in HLA-D homozygous typing cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 12 ·1983-06-00 ·Pages 3758-61

Owerbach D, Lernmark A, Rask L, Peterson PA, Platz P, Svejgaard A

Abstract

Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of genes controlling the association of HLA specificities with a wide variety of diseases.

MeSH Terms
DNA/analysis,genetics DNA Restriction Enzymes Genes Genes, MHC Class II HLA-DR Antigens Homozygote Humans Lymphocytes/immunology Major Histocompatibility Complex Nucleic Acid Hybridization Plasmids Polymorphism, Genetic
Chemicals
HLA-DR Antigens DNA DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Owerbach D
Lernmark A
Rask L
Peterson P A
Platz P
Svejgaard A
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.12.3758
Published
1983-06-00
Pages
3758-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC394130
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.12.3758 PMC Full Text View journal details
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