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PMID: 6306276 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The high leukemogenic potential of Gross passage A murine leukemia virus maps in the region of the genome corresponding to the long terminal repeat and to the 3' end of env.

Journal of virology ·Vol. 47 ·No. 1 ·1983-07-00 ·Pages 24-32

DesGroseillers L, Villemur R, Jolicoeur P

Abstract

The Gross passage A murine leukemia virus (MuLV) is a highly leukemogenic, ecotropic fibrotropic retrovirus. Its genome is similar to that of other nonleukemogenic ecotropic fibrotropic MuLVs but differs at the 3' end and in the long terminal repeat. To determine whether these modifications were related to its leukemogenic potential, we constructed a viral DNA recombinant in vitro with cloned infectious DNA from this highly leukemogenic Gross passage A MuLV and from a weakly leukemogenic endogenous BALB/c B-tropic MuLV. Infectious viruses, recovered after microinjection of murine cells with recombinant DNA, were injected into newborn mice. We show here that the Gross passage A 1.35-kilobase-pair KpnI fragment (harboring part of gp70, all of p15E, and the long terminal repeat) is sufficient to confer a high leukemogenic potential to this recombinant.

MeSH Terms
AKR murine leukemia virus/genetics,pathogenicity Animals Base Sequence DNA, Recombinant DNA, Viral Genes, Viral Leukemia Virus, Murine/genetics Mice Moloney murine leukemia virus/genetics
Chemicals
DNA, Recombinant DNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
DesGroseillers L
Villemur R
Jolicoeur P
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34 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.47.1.24-32.1983
Published
1983-07-00
Pages
24-32
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255191
Subset
IM
Databases
GENBANK
J02197
External Links
PubMed source DOI: 10.1128/JVI.47.1.24-32.1983 PMC Full Text View journal details
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