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PMID: 6306278 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Specific proteolytic fragmentation of p60v-src in transformed cell lysates.

Journal of virology ·Vol. 47 ·No. 1 ·1983-07-00 ·Pages 253-8

Wells SK, Collett MS

Abstract

Work involving the transforming protein, p60v-src, of Rous sarcoma virus has resulted in the extensive characterization of its protein structure and associated phosphotransferase activity. However, in many investigations proteolytic fragments (principally p52v-src) of the src protein are actually studied. Here, we emphasize potential problems in the interpretation of experimental results in which the proteolytic fragmentation of p60v-src may be involved and offer several means for the complete prevention of this p60v-src degradation.

MeSH Terms
Animals Arvicolinae Avian Sarcoma Viruses/genetics Buffers Cell Fractionation Cell Line Cell Transformation, Viral Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Genes, Viral Oncogene Protein pp60(v-src) Phosphotransferases Viral Proteins/isolation & purification
Chemicals
Buffers Viral Proteins Phosphotransferases Oncogene Protein pp60(v-src)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wells S K
Collett M S
References (23)
23 references, click to expand
  1. Immunological identification of two adenovirus 2-induced early proteins possibly involved in cell transformation.
    Nature. 1976 Nov 18;264(5583):263-6 PMID: 1004548
  2. Identification of a transformation-specific antigen induced by an avian sarcoma virus.
    Nature. 1977 Sep 22;269(5626):346-8 PMID: 198667
  3. Protein kinase activity associated with the avian sarcoma virus src gene product.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4 PMID: 205879
  4. Identification of a polypeptide encoded by the avian sarcoma virus src gene.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1567-71 PMID: 206909
  5. Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoprotein.
    Cell. 1978 Oct;15(2):561-72 PMID: 214242
  6. The src gene product of transformed and morphologically reverted ASV-infected mammalian cells.
    Nature. 1979 Sep 20;281(5728):195-8 PMID: 225672
  7. Evidence that the avian sarcoma virus transforming gene product is a cyclic AMP-independent protein kinase.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6260-4 PMID: 230504
  8. Evidence that the src gene product of Rous sarcoma virus is membrane associated.
    Virology. 1980 Feb;101(1):25-40 PMID: 6243822
  9. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.
    Anal Biochem. 1979 Sep 15;98(1):132-5 PMID: 543547
  10. Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosine.
    Nature. 1980 May 15;285(5761):167-9 PMID: 6246443
  11. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 PMID: 6246487
  12. Evidence that the phosphorylation of tyrosine is essential for cellular transformation by Rous sarcoma virus.
    Cell. 1980 Jul;20(3):807-16 PMID: 6251974
  13. Protein phosphorylation mediated by partially purified avian sarcoma virus transforming-gene product.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 2,:907-17 PMID: 6253222
  14. Molecular events in cells transformed by Rous Sarcoma virus.
    J Cell Biol. 1980 NOV;87(2 Pt 1):319-25 PMID: 6253501
  15. The protein encoded by the transforming gene of avian sarcoma virus (pp60src) and a homologous protein in normal cells (pp60proto-src) are associated with the plasma membrane.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3783-7 PMID: 6253989
  16. Differences in intracellular location of pp60src in rat and chicken cells transformed by Rous sarcoma virus.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4142-6 PMID: 6254012
  17. Cellular location of viral transforming proteins.
    Cell. 1980 Oct;21(3):601-2 PMID: 6254662
  18. Structural and functional domains of the Rous sarcoma virus transforming protein (pp60src).
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1624-8 PMID: 6262821
  19. Purification of a tyrosine-specific protein kinase from Rous sarcoma virus-induced rat tumor.
    J Biol Chem. 1982 Jun 25;257(12):7135-42 PMID: 6282832
  20. Evidence the pp60src, the product of the Rous sarcoma virus src gene, undergoes autophosphorylation.
    J Virol. 1982 Jan;41(1):1-7 PMID: 6283105
  21. Changes in amino-terminal sequences of pp60src lead to decreased membrane association and decreased in vivo tumorigenicity.
    Cell. 1982 Apr;28(4):889-96 PMID: 6284379
  22. The transforming proteins of Rous sarcoma virus, Harvey sarcoma virus and Abelson virus contain tightly bound lipid.
    Cell. 1982 Dec;31(2 Pt 1):465-74 PMID: 6297767
  23. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.47.1.253-258.1983
Published
1983-07-00
Pages
253-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255245
Subset
IM
Grants
NCI NIH HHS · 5R01-CA29041-02 · United States
External Links
PubMed source DOI: 10.1128/JVI.47.1.253-258.1983 PMC Full Text View journal details
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