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PMID: 6306281 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of polyoma virus transcription in murine embryonal carcinoma cells.

Journal of virology ·Vol. 47 ·No. 1 ·1983-07-00 ·Pages 55-64

Dandolo L, Blangy D, Kamen R

Abstract

Undifferentiated murine embryonal carcinoma (EC) cells are resistant to infection with wild-type polyoma virus. The block appears to be located at the transcriptional level. Polyoma host range mutants capable of expressing early and late functions in EC cells have been isolated. The modifications responsible for the phenotype of these mutants are localized in the noncoding region of polyoma DNA genome, containing regulatory sequences for replication and transcription. We compared the 5' termini of early and late mRNAs of wild-type polyoma and mutant viruses in EC cells and in permissive cells. Our results show that wild-type mRNA is normally spliced in EC cells but present at a very low level. The sequence modifications of the mutant viruses lead to a 100-fold increase in the production of mRNA in these cells, but the major 5' termini of early and late mRNAs are identical to those in wild-type-infected 3T6 cells.

MeSH Terms

Animals Cell Line DNA Replication DNA, Viral/biosynthesis Electrophoresis, Polyacrylamide Gel Gene Expression Regulation Genes, Viral Mice Mutation Neoplasms, Experimental/microbiology Phenotype Polyomavirus/genetics RNA Splicing Teratoma/microbiology Transcription, Genetic Virus Replication

Chemicals

DNA, Viral

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Dandolo L

Blangy D

Kamen R

References (24)

24 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.47.1.55-64.1983

Published

1983-07-00

Pages

55-64

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC255198

Subset

External Links

PubMed source DOI: 10.1128/JVI.47.1.55-64.1983 PMC Full Text View journal details

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Regulation of polyoma virus transcription in murine embryonal carcinoma cells.

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