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PMID: 6306590 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A new restriction endonuclease from Acetobacter pasteurianus.

Nucleic acids research ·Vol. 11 ·No. 13 ·1983-07-11 ·Pages 4409-15

Seurinck J, Van de Voorde A, Van Montagu M

Abstract

A restriction endonuclease, ApaI, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage lambda DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave phi X174 DNA nor plasmid pBR322 DNA. This enzyme recognizes the sequence (formula; see text) and cuts at the sites indicated by the arrows.

MeSH Terms
Acetobacter/enzymology Base Sequence DNA Restriction Enzymes/isolation & purification,metabolism DNA, Viral Deoxyribonucleases, Type II Site-Specific Plasmids Substrate Specificity
Chemicals
DNA, Viral DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific GGGCCC-specific type II deoxyribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Seurinck J
Van de Voorde A
Van Montagu M
References (4)
4 references, click to expand
  1. Complete nucleotide sequence of SV40 DNA.
    Nature. 1978 May 11;273(5658):113-20 PMID: 205802
  2. Nucleotide sequence of the Hind-C fragment of simian virus 40 DNA. Comparison of the 5'-untranslated region of wild-type virus and of some deletion Mutants.
    Eur J Biochem. 1979 Oct;100(1):51-60 PMID: 226361
  3. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  4. Restriction and modification enzymes and their recognition sequences.
    Nucleic Acids Res. 1983 Jan 11;11(1):r135-67 PMID: 6306557
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.13.4409
Published
1983-07-11
Pages
4409-15
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326055
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.13.4409 PMC Full Text View journal details
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