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PMID: 6307285 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The use of valinomycin, nigericin and trichlorocarbanilide in control of the protonmotive force in Escherichia coli cells.

The Biochemical journal ·Vol. 212 ·No. 1 ·1983-04-15 ·Pages 105-12

Ahmed S, Booth IR

Abstract

Valinomycin, nigericin and trichlorocarbanilide were assessed for their ability to control the protonmotive force in Escherichia coli cells. Valinomycin, at high K+ concentrations, was found to decrease the membrane potential delta phi and indirectly to decrease the pH gradient delta pH. Nigericin was found to have two modes of action. At low concentrations (0.05-2 microM) it carried out K+/H+ exchange and decreased delta pH. At higher concentrations (50 microM) it carried out a K+-dependent transfer of H+, decreasing both delta phi and delta pH. In EDTA-treated cells only the latter mode of action was evident, whereas in a mutant sensitive to deoxycholate both types of effect were observed. Trichlorocarbanilide is proposed as an alternative to nigericin for the specific control of delta pH, and it can be used in cells not treated with EDTA.

MeSH Terms
Anti-Bacterial Agents/pharmacology Biological Transport Carbanilides/pharmacology Edetic Acid/pharmacology Escherichia coli/drug effects,metabolism Hydrogen-Ion Concentration Nigericin/pharmacology Potassium/pharmacology Protons Thermodynamics Valinomycin/pharmacology
Chemicals
Anti-Bacterial Agents Carbanilides Protons Valinomycin Edetic Acid triclocarban Nigericin Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ahmed S
Booth I R
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2120105
Published
1983-04-15
Pages
105-12
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1152016
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2120105 PMC Full Text View journal details
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