Cytochemical demonstration of phosphatases in the rat liver by a cerium-based method in combination with osmium tetroxide and potassium ferrocyanide postfixation.

Histochemistry ·Vol. 78 ·No. 1 ·1983-00-00 ·Pages 71-9

Hulstaert CE, Kalicharan D, Hardonk MJ

Abstract

Acid phosphatase, alkaline phosphatase, glucose-6-phosphatase, Mg-activated adenosine triphosphatase and 5'nucleotidase were demonstrated in the rat liver using a cerium-based method. This method can be applied routinely and yields better results than the lead-based method. The tissue was postfixed in osmium tetroxide and potassium ferrocyanide which considerably enhances the membrane contrast in comparison with solely osmium tetroxide postfixation. This facilitates the precise localization of the reaction product.

MeSH Terms

Acid Phosphatase/metabolism Adenosine Triphosphatases/metabolism Alkaline Phosphatase/metabolism Animals Cerium Fixatives Glucose-6-Phosphatase/metabolism Histocytochemistry Liver/enzymology Male Nucleotidases/metabolism Phosphoric Monoester Hydrolases/metabolism Rats

Chemicals

Fixatives Cerium Nucleotidases Alkaline Phosphatase Acid Phosphatase Phosphoric Monoester Hydrolases Glucose-6-Phosphatase Adenosine Triphosphatases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hulstaert C E

Kalicharan D

Hardonk M J

References (6)

6 references, click to expand

Glucose 6-phosphatase and thiamine pyrophosphatase activities in the jejunal epithelium of the mouse.
J Histochem Cytochem. 1970 May;18(5):361-4 PMID: 4192901
X-ray microanalysis of aldehyde-fixed glycogen contrast-stained by OsVI . FeII and OsVI . RuIV complexes.
J Histochem Cytochem. 1980 Nov;28(11):1242-50 PMID: 6159393
A direct lead method for the electron microscopic visualization of alkaline phosphatase activity.
J Histochem Cytochem. 1966 May;14(5):429-31 PMID: 5961999
Localization of NADH oxidase on the surface of human polymorphonuclear leukocytes by a new cytochemical method.
J Cell Biol. 1975 Dec;67(3):566-86 PMID: 407
Histochemistry of hepatic phosphatases of a physiologic pH; with special reference to the demonstration of bile canaliculi.
Am J Clin Pathol. 1957 Jan;27(1):13-23 PMID: 13410831
A cytochemical method for the demonstration of 5'-nucleotidase in mouse peritoneal macrophages, with cerium ions used as trapping agent.
Histochemistry. 1982;75(4):437-43 PMID: 6294007

Article Info

Journal

Histochemistry

Abbr.

Histochemistry

ISSN

0301-5564

DOI

10.1007/BF00491113

Published

1983-00-00

Pages

71-9

Language

English

Region

Germany

NLM ID

0411300

Subset

External Links

PubMed source DOI: 10.1007/BF00491113

Analysis Services

Cytochemical demonstration of phosphatases in the rat liver by a cerium-based method in combination with osmium tetroxide and potassium ferrocyanide postfixation.

Contact

WeChat Official Account