Amplified DNA in Streptomyces fradiae.

Journal of bacteriology ·Vol. 155 ·No. 2 ·1983-08-00 ·Pages 459-66

Fishman SE, Hershberger CL

Abstract

A spontaneous mutant of Streptomyces fradiae contained an amplifiable unit of DNA with a sequence length of approximately 10.5 kilobases that was amplified to approximately 500 copies per chromosome. The amplified DNA appears to be cryptic. SalI fragments of the amplified DNA were cloned into Escherichia coli to construct a restriction map and characterize the amplified DNA. The amplified DNA contained tandem repeats of the amplifiable unit of DNA. The unit had an average base composition of 71% guanine plus cytosine, similar to the chromosomal DNA of Streptomyces species. At least a portion of the amplifiable unit of DNA was present at a low copy number in the wild-type strain. The phenotype of amplified DNA was designated Ads1SF for amplified DNA sequence 1 in S. fradiae.

MeSH Terms

DNA Restriction Enzymes DNA, Bacterial/analysis DNA, Recombinant/analysis Gene Amplification Mutation Nucleic Acid Hybridization Plasmids Streptomyces/genetics

Chemicals

DNA, Bacterial DNA, Recombinant DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Fishman S E

Hershberger C L

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29 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.155.2.459-466.1983

Published

1983-08-00

Pages

459-66

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC217710

Subset

External Links

PubMed source DOI: 10.1128/jb.155.2.459-466.1983 PMC Full Text View journal details

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