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PMID: 6310356 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Establishment of spontaneously outgrowing lymphoblastoid cell lines with Cyclosporin A.

Medical microbiology and immunology ·Vol. 172 ·No. 2 ·1983-00-00 ·Pages 87-99

von Knebel Doeberitz M, Bornkamm GW, zur Hausen H

Abstract

To establish spontaneously outgrowing EBV (Epstein-Barr virus) transformed lymphoid cell lines from healthy EBV-seropositive adults different culture conditions were investigated. The effect of T-cell depletion was compared with that of adding Cyclosporin A (CSA) to unseparated mononuclear blood cells, either at the onset of cultivation or after 3 weeks. The highest frequency of spontaneous outgrowth of EBV-transformed cells was obtained in unseparated cultures when the addition of CSA was delayed. The presence of unimpaired T cells at the onset of the culture period appeared to have a favourable effect on the spontaneous outgrowth of EBV-carrying lymphoblastoid cell lines.

MeSH Terms
Antigens, Viral/analysis B-Lymphocytes Cell Line Cell Transformation, Viral Cyclosporins/pharmacology Epstein-Barr Virus Nuclear Antigens Herpesvirus 4, Human/immunology,physiology Humans T-Lymphocytes/physiology Virus Replication/drug effects
Chemicals
Antigens, Viral Cyclosporins Epstein-Barr Virus Nuclear Antigens
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
von Knebel Doeberitz M
Bornkamm G W
zur Hausen H
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39 references, click to expand
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Article Info
Journal
Medical microbiology and immunology
Abbr.
Med Microbiol Immunol
ISSN
0300-8584
DOI
10.1007/BF02124509
Published
1983-00-00
Pages
87-99
Language
English
Region
Germany
NLM ID
0314524
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02124509 View journal details
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