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PMID: 6310558 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Encapsidation sequences for spleen necrosis virus, an avian retrovirus, are between the 5' long terminal repeat and the start of the gag gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 19 ·1982-10-00 ·Pages 5986-90

Watanabe S, Temin HM

Abstract

The minimal cis-acting sequences outside the long terminal repeat (LTR) required for formation of an infectious retrovirus cloning vector were determined with recombinants of spleen necrosis virus (SNV) DNA and herpes simplex virus type 1 thymidine kinase gene. The 3' end of SNV DNA was removed to within 40 base pairs (bp) from the 3' LTR with only a 2-fold effect on the recovery of infectious recombinant virus. However, when the 5' end of SNV DNA was removed to within 100 bp from the 5' LTR, infectious recombinant virus was not recovered. Deletion mutants constructed around this latter region showed that nucleotides between 100 and 285 bp from the 5' LTR are necessary for encapsidation of genomic viral RNA. We call this region required for encapsidation E.

MeSH Terms
Animals Antigens, Viral/genetics Base Sequence Capsid/genetics Chick Embryo DNA Restriction Enzymes DNA, Recombinant/analysis Gene Products, gag Genes, Viral Mutation Nucleic Acid Hybridization Plasmids Retroviridae/genetics Transfection Viral Proteins/genetics
Chemicals
Antigens, Viral DNA, Recombinant Gene Products, gag Viral Proteins DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Watanabe S
Temin H M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.19.5986
Published
1982-10-00
Pages
5986-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347036
Subset
IM
Grants
NCI NIH HHS · CA-07175 · United States
NCI NIH HHS · CA-09075 · United States
NCI NIH HHS · CA-22443 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.19.5986 PMC Full Text View journal details
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