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PMID: 6310577 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Metabolism of leukotriene A4 by an enzyme in blood plasma: a possible leukotactic mechanism.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 17 ·1983-09-00 ·Pages 5425-9

Fitzpatrick F, Haeggström J, Granström E, Samuelsson B

Abstract

Cell-free mammalian plasma transformed leukotriene A4 into leukotriene B4. This conversion originated from a soluble enzymatic activity. Heating at 56 degrees C or digestion of plasma with a proteolytic enzyme eliminated formation of leukotriene B4 but not other diastereomeric dihydroxyicosatetraenoic acids formed by nonenzymatic hydrolysis of leukotriene A4. Plasma from several mammals, including guinea pigs, pigs, cows, sheep, rabbits, rats, dogs, and humans, exhibited a qualitatively similar activity. Maximal production of leukotriene B4 occurred in guinea pig plasma near a pH of 7.6; metabolic capacity approached saturation at a substrate concentration of 10 microM. Leukotriene A4 anion was transformed but not its methyl ester. The results suggest that conversion of leukotriene A4 by an enzyme in blood plasma of mammals may provide local gradients of the chemotactic substance leukotriene B4. High interfacial concentrations of leukotriene B4 between the vascular endothelium and leukocytes could facilitate their diapedesis and accumulation at inflammatory sites.

MeSH Terms
Animals Arachidonic Acids/blood Biological Assay Cattle Chemotaxis, Leukocyte Dogs Guinea Pigs Humans Kinetics Leukotriene A4 Lung/drug effects Peptide Hydrolases/blood Rabbits Rats SRS-A/blood,pharmacology Sheep Species Specificity Swine Tritium
Chemicals
Arachidonic Acids Leukotriene A4 SRS-A Tritium Peptide Hydrolases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fitzpatrick F
Haeggström J
Granström E
Samuelsson B
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.17.5425
Published
1983-09-00
Pages
5425-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384269
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.17.5425 PMC Full Text View journal details
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