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PMID: 6311180 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The mechanism of adenosine production in rat polymorphonuclear leucocytes.

The Biochemical journal ·Vol. 214 ·No. 2 ·1983-08-15 ·Pages 325-30

Worku Y, Newby AC

Abstract

Adenosine production in intact rat polymorphonuclear leucocytes was studied during 2-deoxyglucose-induced ATP catabolism. A cell-free system containing the cytosolic 5'-nucleotidase (EC 3.1.3.5) as the only phosphohydrolase was also studied. The rate of adenosine formation in both intact cells and the cell-free system showed a similar dependence on energy charge (([ATP] + 1/2 [ADP]/([ATP] + [ADP] + [AMP])), being maximal only at values close to 0.8. Sufficient cytosolic 5'-nucleotidase was present in intact cells to explain the observed rate of adenosine formation. We conclude that the cytosolic 5'-nucleotidase is responsible for adenosine production in rat polymorphonuclear leucocytes. This mechanism provides a direct biochemical link between the energy status of a cell and the rate of adenosine formation.

MeSH Terms

5'-Nucleotidase Adenosine/biosynthesis Adenosine Monophosphate/blood Adenosine Triphosphate/blood Animals Cell-Free System Cytosol/enzymology Hypoxanthine Hypoxanthines/biosynthesis Inosine Monophosphate/blood Liver/enzymology Neutrophils/enzymology,metabolism Nucleotidases/metabolism Rats

Chemicals

Hypoxanthines Inosine Monophosphate Hypoxanthine Adenosine Monophosphate Adenosine Triphosphate Nucleotidases 5'-Nucleotidase Adenosine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Worku Y

Newby A C

References (26)

26 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2140325

Published

1983-08-15

Pages

325-30

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1152251

Subset

External Links

PubMed source DOI: 10.1042/bj2140325 PMC Full Text View journal details

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The mechanism of adenosine production in rat polymorphonuclear leucocytes.

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