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PMID: 6311809 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning of genes for naphthalene metabolism in Pseudomonas putida.

Journal of bacteriology ·Vol. 156 ·No. 1 ·1983-10-00 ·Pages 89-94

Grund AD, Gunsalus IC

Abstract

Plasmid pIG7 DNA cloned in Pseudomonas putida with the broad-host-range vectors pRK290 and pKT240 expresses the genes encoding nephthalene oxidation in the presence of the intermediate substrate, salicylate, or the gratuitous inducer, anthranilate. Two operons, nahAF and nahGK, cloned from the EcoRI fragment A (25 kilobases) are under wild-type regulation by the nahR locus. Deletion plasmids provide a restriction map of both operons. Double transformants containing structural and regulatory cistron nahR in trans are used to demonstrate positive control of expression.

MeSH Terms
Cloning, Molecular DNA Restriction Enzymes Deoxyribonuclease EcoRI Genes, Bacterial Genes, Regulator Naphthalenes/metabolism Operon Oxidation-Reduction Pseudomonas/genetics
Chemicals
Naphthalenes naphthalene DNA Restriction Enzymes Deoxyribonuclease EcoRI
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Grund A D
Gunsalus I C
References (24)
24 references, click to expand
  1. The metabolic divergence in the meta cleavage of catechols by Pseudomonas putida NCIB 10015. Physiological significance and evolutionary implications.
    Eur J Biochem. 1972 Jul 24;28(3):347-56 PMID: 4342908
  2. Genetic basis of the biodegradation of salicylate in Pseudomonas.
    J Bacteriol. 1972 Nov;112(2):815-23 PMID: 4628746
  3. Transmissible plasmid coding early enzymes of naphthalene oxidation in Pseudomonas putida.
    J Bacteriol. 1973 Jun;114(3):974-9 PMID: 4712575
  4. A transmissible plasmid controlling camphor oxidation in Pseudomonas putida.
    Proc Natl Acad Sci U S A. 1973 Mar;70(3):885-9 PMID: 4351810
  5. Regulation: positive control.
    Annu Rev Genet. 1974;8:219-42 PMID: 4374117
  6. Metabolism of toluene and xylenes by Pseudomonas (putida (arvilla) mt-2: evidence for a new function of the TOL plasmid.
    J Bacteriol. 1975 Oct;124(1):7-13 PMID: 1176436
  7. Naphthalene metabolism by pseudomonads: the oxidation of 1,2-dihydroxynaphthalene to 2-hydroxychromene-2-carboxylic acid and the formation of 2'-hydroxybenzalpyruvate.
    Biochem Biophys Res Commun. 1976 Oct 4;72(3):1116-21 PMID: 985513
  8. Plasmids in Pseudomonas.
    Annu Rev Genet. 1976;10:7-30 PMID: 797315
  9. Isolation of metabolic plasmid DNA from Pseudomonas putida.
    Biochem Biophys Res Commun. 1977 Mar 7;75(1):13-9 PMID: 849300
  10. RSF1010 plasmid as a potentially useful vector in Pseudomonas species.
    J Bacteriol. 1978 Mar;133(3):1527-9 PMID: 417070
  11. Isolation of TOL and RP4 recombinants by integrative suppression.
    J Bacteriol. 1978 Apr;134(1):270-7 PMID: 418059
  12. Transposition of Pseudomonas toluene-degrading genes and expression in Escherichia coli.
    Nature. 1978 Jul 13;274(5667):179-80 PMID: 96356
  13. Restriction endonuclease sites and aromatic metabolic plasmid structure.
    Biochem Biophys Res Commun. 1978 May 30;82(2):411-6 PMID: 666852
  14. NIC, a conjugative nicotine-nicotinate degradative plasmid in Pseudomonas convexa.
    J Bacteriol. 1978 Jul;135(1):289-90 PMID: 670150
  15. A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance.
    Mol Gen Genet. 1979;177(1):65-72 PMID: 231729
  16. Construction of a partial diploid for the degradative pathway encoded by the TOL plasmid (pWWO) from Pseudomonas putida mt-2: evidence for the positive nature of the regulation by the xyIR gene.
    Mol Gen Genet. 1980 Jan;177(2):321-8 PMID: 6929031
  17. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51 PMID: 7012838
  18. Molecular cloning of gene xylS of the TOL plasmid: evidence for positive regulation of the xylDEGF operon by xylS.
    J Bacteriol. 1981 Nov;148(2):413-8 PMID: 6271729
  19. Host: vector systems for gene cloning in Pseudomonas.
    Curr Top Microbiol Immunol. 1982;96:47-67 PMID: 6276095
  20. Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7458-62 PMID: 6950388
  21. Plasmid gene organization: naphthalene/salicylate oxidation.
    Proc Natl Acad Sci U S A. 1982 Feb;79(3):874-8 PMID: 6278499
  22. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.
    Gene. 1981 Dec;16(1-3):237-47 PMID: 6282695
  23. Cloning and expression in Escherichia coli of the naphthalene degradation genes from plasmid NAH7.
    J Bacteriol. 1983 Feb;153(2):822-9 PMID: 6296054
  24. Transduction of linked genetic characters of the host by bacteriophage P1.
    Virology. 1955 Jul;1(2):190-206 PMID: 13267987
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.156.1.89-94.1983
Published
1983-10-00
Pages
89-94
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215054
Subset
IM
Grants
NIAID NIH HHS · AI16193 · United States
NIGMS NIH HHS · GM07750 · United States
External Links
PubMed source DOI: 10.1128/jb.156.1.89-94.1983 PMC Full Text View journal details
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