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PMID: 6312066 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Salt-resistant association of simian virus 40 T antigen with simian virus 40 DNA in nucleoprotein complexes.

Journal of virology ·Vol. 47 ·No. 2 ·1983-08-00 ·Pages 276-86

Rudolph K, Mann K

Abstract

Treatment of nucleoprotein complexes (NPCs) from simian virus 40 (SV40)-infected TC7 cells with NaCl (1 or 2 M) or guanidine-hydrochloride (1 or 2 M) resulted in a significant fraction of T antigen still associated with SV40 (I) DNA. Immunoprecipitation of the salt-treated NPCs with SV40 anti-T serum indicated that T antigen is preferentially associated with SV40 (I) DNA rather than with SV40 (II) DNA. Treatment of the NPCs with 4 M guanidine-hydrochloride, however, resulted in a substantial decrease in the amount of SV40 (I) and (II) DNA associated with T antigen. As the temperature was increased to 37 degrees C during incubation of NPCs with NaCl or guanidine-hydrochloride, there was a decrease in the amount of SV40 (I) and (II) DNA immunoprecipitated with SV40 anti-T serum. In the absence of salt, temperature had no effect on the association of T antigen with the SV40 DNA in the NPCs. Treatment of NPCs from SV40 wildtype or tsA58-infected cells grown at the permissive temperature with 1 or 2 M NaCl indicated that tsA T antigen has the same sensitivities as wild-type T antigen to high salt treatment when bound to DNA in NPCs. Characterization of the proteins associated with SV40 (I) DNA after high salt treatment revealed that, in addition to T antigen, a certain amount of viral capsid proteins VP1 and VP3 remained associated with the DNA. Complexes containing SV40 (I) DNA had a sedimentation value of 53S after 1 M NaCl treatment and 43S after 2 M NaCl treatment.

MeSH Terms
Antigens, Viral, Tumor Centrifugation, Density Gradient DNA, Viral/analysis Deoxyribonuclease I Deoxyribonucleoproteins/analysis Endodeoxyribonucleases Guanidine Guanidines/pharmacology Precipitin Tests Simian virus 40/analysis,immunology Sodium Chloride/pharmacology
Chemicals
Antigens, Viral, Tumor DNA, Viral Deoxyribonucleoproteins Guanidines Sodium Chloride Endodeoxyribonucleases Deoxyribonuclease I Guanidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rudolph K
Mann K
References (51)
51 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.47.2.276-286.1983
Published
1983-08-00
Pages
276-86
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255259
Subset
IM
Grants
NCI NIH HHS · CA-26048 · United States
External Links
PubMed source DOI: 10.1128/JVI.47.2.276-286.1983 PMC Full Text View journal details
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