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PMID: 6313628 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning with bifunctional plasmid vectors in Bacillus subtilis: isolation of a spontaneous mutant of Bacillus subtilis with enhanced transformability for Escherichia coli-propagated chimeric plasmid DNA.

Journal of bacteriology ·Vol. 156 ·No. 2 ·1983-11-00 ·Pages 934-6

Ostroff GR, Pène JJ

Abstract

Hybrid plasmid DNA cloned in Escherichia coli undergoes deletions when returned to competent Bacillus subtilis, even in defined restriction and modification mutants of strain 168. We have isolated a mutant of B. subtilis MI112 which is stably transformed at high frequency by chimeric plasmid DNA propagated in E. coli.

MeSH Terms

Bacillus subtilis/genetics,isolation & purification Cloning, Molecular Escherichia coli/genetics Genetic Vectors Genotype Mutation Nucleic Acid Hybridization Phenotype Plasmids Species Specificity Transformation, Bacterial

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ostroff G R

Pène J J

References (5)

5 references, click to expand

The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis.
Mol Gen Genet. 1978 Nov 9;166(3):259-67 PMID: 105241
Restriction of plasmid-mediated transformation in Bacillus subtilis 168.
Mol Gen Genet. 1979 Sep;175(2):235-7 PMID: 117281
Construction of a colony bank of E. coli containing hybrid plasmids representative of the Bacillus subtilis 168 genome. Expression of functions harbored by the recombinant plasmids in B. subtilis.
Mol Gen Genet. 1979 Oct 3;176(2):239-45 PMID: 119126
Molecular cloning and expression of Bacillus licheniformis beta-lactamase gene in Escherichia coli and Bacillus subtilis.
J Bacteriol. 1981 Jan;145(1):422-8 PMID: 6257638
Plasmid transformation in Bacillus subtilis: fate of plasmid DNA.
Mol Gen Genet. 1981;181(4):424-33 PMID: 6790906

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.156.2.934-936.1983

Published

1983-11-00

Pages

934-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC217920

Subset

Grants

NIAID NIH HHS · AI-11868 · United States

External Links

PubMed source DOI: 10.1128/jb.156.2.934-936.1983 PMC Full Text View journal details

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Molecular cloning with bifunctional plasmid vectors in Bacillus subtilis: isolation of a spontaneous mutant of Bacillus subtilis with enhanced transformability for Escherichia coli-propagated chimeric plasmid DNA.

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