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PMID: 6314279 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of soybean chloroplast DNA regions which contain the psb A and trn H genes and cover the ends of the large single copy region and one end of the inverted repeats.

Nucleic acids research ·Vol. 11 ·No. 20 ·1983-10-25 ·Pages 7157-67

Spielmann A, Stutz E

Abstract

The soybean chloroplast psb A gene (photosystem II thylakoid membrane protein of Mr 32 000, lysine-free) and the trn H gene (tRNAHisGUG), which both map in the large single copy region adjacent to one of the inverted repeat structures (IR1), have been sequenced including flanking regions. The psb A gene shows in its structural part 92% sequence homology with the corresponding genes of spinach and N. debneyi and contains also an open reading frame for 353 aminoacids. The aminoacid sequence of a potential primary translation product (calculated Mr, 38 904, no lysine) diverges from that of spinach and N. debneyi in only two positions in the C-terminal part. The trn H gene has the same polarity as the psb A gene and the coding region is located at the very end of the large single copy region. The deduced sequence of the soybean chloroplast tRNAHisGUG is identical with that of Zea mays chloroplasts. Both ends of the large single copy region were sequenced including a small segment of the adjacent IR1 and IR2.

MeSH Terms
Amino Acid Sequence Chloroplasts/metabolism DNA/genetics DNA Restriction Enzymes Genes Molecular Weight Nucleic Acid Conformation Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins/genetics Plants/genetics Soybeans
Chemicals
Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Spielmann A
Stutz E
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17 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.20.7157
Published
1983-10-25
Pages
7157-67
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326446
Subset
IM
Databases
GENBANK
K01756, X00152
External Links
PubMed source DOI: 10.1093/nar/11.20.7157 PMC Full Text View journal details
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