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PMID: 6315407 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Deletion of Cmu genes in mouse B lymphocytes upon stimulation with LPS.

The EMBO journal ·Vol. 2 ·No. 11 ·1983-00-00 ·Pages 1929-35

Radbruch A, Sablitzky F

Abstract

Mouse B lymphocytes can be activated polyclonally by bacterial lipopolysaccharide (LPS) to differentiate into plasmablasts. Within several days many cells perform immunoglobulin (Ig) class switching in vitro. We have purified LPS blasts expressing IgM or only IgG3 on the cell surface and analysed the DNA of these cells by Southern hybridisation blotting to detect rearrangement or deletion of CH genes. Quantitative evaluation of the Southern blots suggests that populations of surface IgG3+ (sIgG3+) cells from 6-day and sIgM+ cells from 8-day-old cultures contain only about half as many Cmu genes as spleen cells. Cmu deletion is nearly complete in populations of sIgG3+ cells from 9-day-old cultures. Therefore, upon stimulation with LPS, within a few days Cmu is deleted in most sIgG3+ cells from both chromosomes.

MeSH Terms
Animals B-Lymphocytes/immunology Cell Separation Chromosome Deletion DNA Restriction Enzymes Flow Cytometry Genes Immunoglobulin Constant Regions/genetics Immunoglobulin Heavy Chains/genetics Immunoglobulin gamma-Chains/genetics Immunoglobulin mu-Chains/genetics Immunoglobulins/genetics Lipopolysaccharides/immunology Mice
Chemicals
Immunoglobulin Constant Regions Immunoglobulin Heavy Chains Immunoglobulin gamma-Chains Immunoglobulin mu-Chains Immunoglobulins Lipopolysaccharides DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Radbruch A
Sablitzky F
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35 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1929-35
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555390
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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