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PMID: 6315411 Published · ppublish English Journal Article

Purification and partial amino acid sequence analysis of the cellular tumour antigen, p53, from mouse SV40-transformed cells.

The EMBO journal ·Vol. 2 ·No. 11 ·1983-00-00 ·Pages 1993-9

Leppard K, Totty N, Waterfield M, Harlow E, Jenkins J, Crawford L

Abstract

The cellular tumour antigen p53 is implicated in the transformation process. To compare p53 from transformed cells and their normal counterparts in detail, and so to identify any structural differences that might alter p53 function, requires information on the primary structure of the protein. By making use of immunochemical techniques we have been able to purify nanomole amounts of p53. This was sufficient, using high sensitivity automated gas-phase sequencing techniques to determine the amino acid sequence of two tryptic peptides from p53. Their sequences agree completely with the predicted polypeptide sequence derived from a cloned cDNA for p53 mRNA and provide the first data on the amino acid sequence of p53. A combination of the high sensitivity amino acid sequencing procedures used here and cDNA sequence analysis should provide the complete amino acid sequence of p53.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Antigens, Neoplasm/genetics,isolation & purification Base Sequence Cell Line Cell Transformation, Viral DNA/genetics Simian virus 40/genetics
Chemicals
Amino Acids Antigens, Neoplasm DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Leppard K
Totty N
Waterfield M
Harlow E
Jenkins J
Crawford L
References (28)
28 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Microinjection of monoclonal antibody to protein p53 inhibits serum-induced DNA synthesis in 3T3 cells.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6309-12 PMID: 6292898
  3. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
    J Immunol. 1975 Dec;115(6):1617-24 PMID: 1102604
  4. Extraction and fingerprint analysis of simian virus 40 large and small T-antigens.
    J Virol. 1978 Oct;28(1):140-53 PMID: 212599
  5. T antigen is bound to a host protein in SV40-transformed cells.
    Nature. 1979 Mar 15;278(5701):261-3 PMID: 218111
  6. Detection of a transformation-related antigen in chemically induced sarcomas and other transformed cells of the mouse.
    Proc Natl Acad Sci U S A. 1979 May;76(5):2420-4 PMID: 221923
  7. Characterization of a 54K dalton cellular SV40 tumor antigen present in SV40-transformed cells and uninfected embryonal carcinoma cells.
    Cell. 1979 May;17(1):43-52 PMID: 222475
  8. Simian virus 40-transformed cells express new species of proteins precipitable by anti-simian virus 40 tumor serum.
    J Virol. 1979 Aug;31(2):472-83 PMID: 225566
  9. Association of a murine 53,000-dalton phosphoprotein with simian virus 40 large-T antigen in transformed cells.
    J Virol. 1980 Apr;34(1):213-24 PMID: 6246255
  10. Relationship among Tau antigens isolated from various lines of simian virus 40-transformed cells.
    J Virol. 1980 Jun;34(3):650-7 PMID: 6247503
  11. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens.
    J Virol. 1980 Jun;34(3):752-63 PMID: 6155477
  12. Purification and biochemical characterization of the Epstein-Barr virus-determined nuclear antigen and an associated protein with a 53,000-dalton subunit.
    J Virol. 1980 Sep;35(3):592-602 PMID: 6158579
  13. A common transformation-related protein in murine sarcomas and leukemias.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:659-64 PMID: 6933048
  14. Abelson murine leukemia virus-induced tumors elicit antibodies against a host cell protein, P50.
    J Virol. 1980 Nov;36(2):547-55 PMID: 6159484
  15. p53 transformation-related protein: detection by monoclonal antibody in mouse and human cells.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1695-9 PMID: 6940183
  16. Detection of a common feature in several human tumor cell lines--a 53,000-dalton protein.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):41-5 PMID: 6264441
  17. SV40-53K antigen: a possible role for 53K in normal cells.
    Virology. 1981 Jul 30;112(2):785-8 PMID: 6266152
  18. A gas-liquid solid phase peptide and protein sequenator.
    J Biol Chem. 1981 Aug 10;256(15):7990-7 PMID: 7263636
  19. Monoclonal antibodies specific for simian virus 40 tumor antigens.
    J Virol. 1981 Sep;39(3):861-9 PMID: 6169844
  20. Phosphorylation patterns of tumour antigens in cells lytically infected or transformed by simian virus 40.
    J Virol. 1981 Oct;40(1):28-44 PMID: 6270381
  21. Adenovirus E1b-58kd tumor antigen and SV40 large tumor antigen are physically associated with the same 54 kd cellular protein in transformed cells.
    Cell. 1982 Feb;28(2):387-94 PMID: 6277513
  22. A 53-kilodalton protein common to chemically and virally transformed cells shows extensive sequence similarities between species.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):287-91 PMID: 6952184
  23. A monoclonal antibody detecting the adenovirus type 5-E1b-58Kd tumor antigen: characterization of the E1b-58Kd tumor antigen in adenovirus-infected and -transformed cells.
    Virology. 1982 Jul 30;120(2):510-7 PMID: 7048730
  24. Purification of polyoma virus medium-size tumor antigen by immunoaffinity chromatography.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4025-9 PMID: 6287461
  25. Antibodies of predetermined specificity for the NH2 terminus of a cellular protein p53 react with the native molecule: evidence for the presence of different p53s.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1199-203 PMID: 6298784
  26. p53, a transformation-related cellular-encoded protein, can be used as a biochemical marker for the detection of primary mouse tumor cells.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2613-7 PMID: 6189126
  27. Radioimmunoassay of the cellular protein p53 in mouse and human cell lines.
    EMBO J. 1982;1(9):1055-62 PMID: 6765238
  28. Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.
    J Biol Chem. 1971 Oct 25;246(20):6328-34 PMID: 5127429
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1993-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555399
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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