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PMID: 6316111 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Transfer of the delta (argF-lac)U169 mutation between Escherichia coli strains by selection for a closely linked Tn10 insertion.

Molecular & general genetics : MGG ·Vol. 192 ·No. 1-2 ·1983-00-00 ·Pages 293-4

Schweizer H, Boos W

Abstract

To facilitate construction of mutants harboring delta lac for use in gene fusion studies, strains were constructed that carry the transposon Tn10 next to the well defined lac deletion U169. This deletion can now be moved to other Escherichia coli strains in transductional or conjugational crosses by selecting resistance to tetracycline.

MeSH Terms
Chromosome Deletion DNA Transposable Elements Escherichia coli/genetics Genes, Bacterial Lac Operon Mutation
Chemicals
DNA Transposable Elements
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schweizer H
Boos W
References (11)
11 references, click to expand
  1. Phosphate-controlled gene expression in Escherichia coli K12 using Mudl-directed lacZ fusions.
    J Mol Biol. 1982 Jul 5;158(3):347-63 PMID: 6813506
  2. Positive selection for loss of tetracycline resistance.
    J Bacteriol. 1980 Aug;143(2):926-33 PMID: 6259126
  3. Linkage map of Escherichia coli K-12, edition 6.
    Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
  4. Location and orientation of the phoA locus on the Escherichia coli K-12 linkage map.
    J Bacteriol. 1971 Sep;107(3):683-9 PMID: 4937782
  5. CHROMOSOMAL ABERRATIONS ASSOCIATED WITH MUTATIONS TO BACTERIOPHAGE RESISTANCE IN ESCHERICHIA COLI.
    J Bacteriol. 1965 Jan;89:28-40 PMID: 14255678
  6. A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery.
    Cell. 1982 Nov;31(1):227-35 PMID: 6297749
  7. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.
    J Bacteriol. 1980 Aug;143(2):971-80 PMID: 6162838
  8. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.
    J Mol Biol. 1977 Oct 15;116(1):125-59 PMID: 338917
  9. ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI.
    J Biol Chem. 1964 Sep;239:3098-105 PMID: 14217902
  10. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530-3 PMID: 159458
  11. Selection for loss of tetracycline resistance by Escherichia coli.
    J Bacteriol. 1981 Feb;145(2):1110-1 PMID: 7007341
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327683
Published
1983-00-00
Pages
293-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327683
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