Home LiteratureArticle Details
PMID: 6316266 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate.

Nucleic acids research ·Vol. 11 ·No. 21 ·1983-11-11 ·Pages 7631-48

Gorman CM, Howard BH, Reeves R

Abstract

We have studied the effects of sodium butyrate on DNA-mediated gene transfer in an effort to investigate interrelationships between chromatin structure and expression of recombinant plasmids. Our results demonstrate that butyrate affects the early stages of gene activity following DNA uptake at least two levels. First, the number of cells able to express foreign DNA increases from 10% to up to 40%. Second, there is an increase in enhancer-dependent transcription, approximately 30 fold in HeLa cells, involving the SV40 early promoter. Stable transformation efficiencies increase to 4% and 10% in HeLa S3 and monkey kidney CV-1 cells, respectively. Finally, expression of integrated recombinant plasmid genes is reinducible by a second treatment five weeks after initial exposure to this agent.

MeSH Terms
Butyrates/pharmacology Butyric Acid Cell Cycle DNA, Recombinant/metabolism DNA, Viral/genetics Genes/drug effects Genes, Viral/drug effects HeLa Cells/drug effects,metabolism Humans Plasmids/drug effects Simian virus 40/genetics Transcription, Genetic/drug effects
Chemicals
Butyrates DNA, Recombinant DNA, Viral Butyric Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gorman C M
Howard B H
Reeves R
References (54)
54 references, click to expand
  1. Template structural requirements for transcription in vivo by RNA polymerase II.
    Mol Cell Biol. 1982 Dec;2(12):1595-607 PMID: 14582200
  2. Linear DNA does not form chromatin containing regularly spaced nucleosomes.
    Mol Cell Biol. 1982 Dec;2(12):1608-18 PMID: 14582201
  3. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21 PMID: 5231757
  4. Morphology and growth rate changes in Chinese hamster cells cultured in presence of sodium butyrate.
    Exp Cell Res. 1973 Apr;78(2):456-60 PMID: 4349017
  5. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  6. Enzymatic modifications and their possible roles in regulating the binding of basic proteins to DNA and in controlling chromosomal structure.
    Cold Spring Harb Symp Quant Biol. 1974;38:803-19 PMID: 4524789
  7. Growth inhibition and morphological changes caused by lipophilic acids in mammalian cells.
    Proc Natl Acad Sci U S A. 1973 Aug;70(8):2457-61 PMID: 4365381
  8. Butyric acid, a potent inducer of erythroid differentiation in cultured erythroleukemic cells.
    Cell. 1975 Jul;5(3):319-22 PMID: 1056809
  9. Regulation of growth and morphological modulation of HeLa65 cells in monolayer culture by dibutyryl cyclic AMP, butyrate and their analogs.
    J Cell Physiol. 1975 Dec;86 Suppl 2(3 Pt 2):663-72 PMID: 172521
  10. Production of human chorionic gonadotropin in HeLa cell cultures.
    Nature. 1976 Feb 5;259(5542):416-7 PMID: 1250386
  11. Deficiency in histone acetylation in nontransforming host range mutants of polyoma virus.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1092-6 PMID: 177973
  12. Steroid receptors: elements for modulation of eukaryotic transcription.
    Annu Rev Biochem. 1976;45:721-46 PMID: 183602
  13. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  14. Effect of n-butyrate on DNA synthesis in chick fibroblasts and HeLa cells.
    Cell. 1977 Nov;12(3):855-60 PMID: 562721
  15. Structure of chromatin containing extensively acetylated H3 and H4.
    Cell. 1978 Apr;13(4):691-9 PMID: 657272
  16. Sodium butyrate inhibits histone deacetylation in cultured cells.
    Cell. 1978 May;14(1):105-13 PMID: 667927
  17. The transfer and stable integration of the HSV thymidine kinase gene into mouse cells.
    Cell. 1978 May;14(1):133-41 PMID: 208776
  18. Butyrate suppression of histone deacetylation leads to accumulation of multiacetylated forms of histones H3 and H4 and increased DNase I sensitivity of the associated DNA sequences.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2239-43 PMID: 276864
  19. Acetylated histone H4 is preferentially associated with template-active chromatin.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3574-7 PMID: 278972
  20. Uncoupled synthesis of histones and DNA during Friend cell differentiation.
    Nature. 1978 Nov 16;276(5685):276-7 PMID: 280756
  21. Mapping temperature-sensitive and host-range mutations of adenovirus type 5 by marker rescue.
    Virology. 1978 Nov;91(1):39-50 PMID: 726266
  22. Sites in simian virus 40 chromatin which are preferentially cleaved by endonucleases.
    Cell. 1978 Dec;15(4):1511-8 PMID: 215331
  23. Multiacetylated forms of H4 are found in a putative transcriptionally competent chromatin fraction from trout testis.
    Nucleic Acids Res. 1979 Jan;6(1):259-74 PMID: 424292
  24. Sodium butyrate induces new gene expression in Friend erythroleukemic cells.
    J Biol Chem. 1979 May 25;254(10):4283-90 PMID: 285931
  25. A stretch of "late" SV40 viral DNA about 400 bp long which includes the origin of replication is specifically exposed in SV40 minichromosomes.
    Cell. 1979 Feb;16(2):453-66 PMID: 222461
  26. Histone acetylation in CV-1 cells infected with simian virus 40.
    Virology. 1979 Jul 30;96(2):564-75 PMID: 223317
  27. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  28. A nucleosome-free region in SV40 minichromosomes.
    Nature. 1980 May 22;285(5762):263-5 PMID: 6246449
  29. Absence of nucleosomes in a fraction of SV40 chromatin between the origin of replication and the region coding for the late leader RNA.
    Cell. 1980 May;20(1):65-73 PMID: 6248237
  30. Butyrate suppression of position-effect variegation in Drosophila melanogaster.
    Mol Gen Genet. 1980;178(2):465-9 PMID: 6771490
  31. Concentration-dependent effects of sodium butyrate in Chinese hamster cells: cell-cycle progression, inner-histone acetylation, histone H1 dephosphorylation, and induction of an H1-like protein.
    Biochemistry. 1980 Jun 10;19(12):2656-71 PMID: 7397096
  32. Expression of a bacterial gene in mammalian cells.
    Science. 1980 Sep 19;209(4463):1422-7 PMID: 6251549
  33. Genetic and physical linkage of exogenous sequences in transformed cells.
    Cell. 1980 Nov;22(1 Pt 1):309-17 PMID: 6253083
  34. The effect of histone hyperacetylation on the nuclease sensitivity and the solubility of chromatin.
    J Biol Chem. 1981 Apr 10;256(7):3313-8 PMID: 6259161
  35. In vivo sequence requirements of the SV40 early promotor region.
    Nature. 1981 Mar 26;290(5804):304-10 PMID: 6259538
  36. Simian virus 40 tandem repeated sequences as an element of the early promoter.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):943-7 PMID: 6262784
  37. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2072-6 PMID: 7017722
  38. Activation of SV40 genome by 72-base pair tandem repeats of Moloney sarcoma virus.
    Nature. 1982 Feb 18;295(5850):568-72 PMID: 6276775
  39. Accessibility of newly synthesized chromatin to histone acetylase.
    J Biol Chem. 1982 Apr 10;257(7):3945-9 PMID: 7061519
  40. Mechanisms of DNA uptake by mammalian cells: fate of exogenously added DNA monitored by the use of fluorescent dyes.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):422-6 PMID: 6952193
  41. Mechanisms of DNA entry into mammalian cells. II. Phagocytosis of calcium phosphate DNA co-precipitate visualized by electron microscopy.
    Exp Cell Res. 1982 May;139(1):223-34 PMID: 7084316
  42. Histone acetylation increases the solubility of chromatin and occurs sequentially over most of the chromatin. A novel model for the biological role of histone acetylation.
    J Biol Chem. 1982 Jul 10;257(13):7336-47 PMID: 7085629
  43. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  44. Induction of thymidine kinase in enzyme-deficient Chinese hamster cells.
    Cell. 1982 Jun;29(2):483-92 PMID: 6180833
  45. Chromosomal position effects determine transcriptional potential of integrated mammary tumor virus DNA.
    J Mol Biol. 1982 Apr 15;156(3):549-65 PMID: 6288960
  46. Host-specific activation of transcription by tandem repeats from simian virus 40 and Moloney murine sarcoma virus.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6453-7 PMID: 6292901
  47. Alteration in the simian virus 40 maturation pathway after butyrate-induced hyperacetylation of histones.
    J Virol. 1982 Dec;44(3):958-62 PMID: 6294343
  48. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  49. The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6777-81 PMID: 6294651
  50. Transcription of DNA injected into Xenopus oocytes is influenced by template topology.
    Nature. 1983 Mar 3;302(5903):38-43 PMID: 6828158
  51. Transcriptional 'enhancers' from SV40 and polyoma virus show a cell type preference.
    Nucleic Acids Res. 1982 Dec 20;10(24):7965-76 PMID: 6298703
  52. Enhanced transformation by a simian virus 40 recombinant virus containing a Harvey murine sarcoma virus long terminal repeat.
    Mol Cell Biol. 1983 Mar;3(3):325-39 PMID: 6302472
  53. Glucocorticoids and chromosomal position modulate murine mammary tumor virus transcription by affecting efficiency of promoter utilization.
    Mol Cell Biol. 1983 Apr;3(4):551-61 PMID: 6304497
  54. High efficiency DNA-mediated transformation of primate cells.
    Science. 1983 Aug 5;221(4610):551-3 PMID: 6306768
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.21.7631
Published
1983-11-11
Pages
7631-48
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326508
Subset
IM
Grants
NIGMS NIH HHS · GM27602 · United States
External Links
PubMed source DOI: 10.1093/nar/11.21.7631 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2