Home LiteratureArticle Details
PMID: 6316322 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Targeted selection of recombinant clones through gene dosage effects.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 22 ·1983-11-00 ·Pages 6750-4

Rine J, Hansen W, Hardeman E, Davis RW

Abstract

The availability of multicopy plasmid vectors for the yeast Saccharomyces cerevisiae allows the selective amplification of individual segments of the genome. Increased dosage of particular genes results in overproduction of specific gene products and thereby confers resistance to certain metabolic inhibitors. Advantage was taken of this fact to isolate recombinant clones that increase the activities of the enzymes UDP-N-acetylglucosamine-1-P transferase and 3-hydroxy-3-methylglutaryl-CoA reductase.

MeSH Terms
Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Genes Genes, Fungal Genetic Vectors Hydroxymethylglutaryl CoA Reductases/genetics Phosphotransferases/genetics Plasmids Saccharomyces cerevisiae/genetics Transferases (Other Substituted Phosphate Groups)
Chemicals
DNA, Recombinant Hydroxymethylglutaryl CoA Reductases Phosphotransferases Transferases (Other Substituted Phosphate Groups) UDP-N-acetylglucosamine-lysosomal-enzyme-N-acetylglucosaminephosphotransferase DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rine J
Hansen W
Hardeman E
Davis R W
References (31)
31 references, click to expand
  1. Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):2119-23 PMID: 6246523
  2. Transposable elements associated with constitutive expression of yeast alcohol dehydrogenase II.
    Cell. 1981 Feb;23(2):605-14 PMID: 6258806
  3. Micro assay for 3-hydroxy-3-methylglutaryl-CoA reductase in rat liver and in L-cell fibroblasts.
    Biochim Biophys Acta. 1974 Dec 29;370(2):369-77 PMID: 4441486
  4. Tunicamycin--an inhibitor of yeast glycoprotein synthesis.
    Biochem Biophys Res Commun. 1974 May 7;58(1):287-95 PMID: 4598444
  5. Biosynthesis and characterization of large dolichyl diphosphate-linked oligosaccharides in Saccharomyces cerevisiae.
    Biochim Biophys Acta. 1978 Mar 1;539(2):218-29 PMID: 343819
  6. Genetic map of Saccharomyces cerevisiae.
    Microbiol Rev. 1980 Dec;44(4):519-71 PMID: 7010111
  7. Functional genetic expression of eukaryotic DNA in Escherichia coli.
    Proc Natl Acad Sci U S A. 1976 May;73(5):1471-5 PMID: 775490
  8. The biosynthesis of carbamoyl phosphate in Saccharomyces cerevisiae.
    J Gen Microbiol. 1965 Jul;40(1):127-42 PMID: 5856369
  9. Control of cell type in yeast by the mating type locus. The alpha 1-alpha 2 hypothesis.
    J Mol Biol. 1981 Apr 15;147(3):357-72 PMID: 7031257
  10. Competitive inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase by ML-236A and ML-236B fungal metabolites, having hypocholesterolemic activity.
    FEBS Lett. 1976 Dec 31;72(2):323-6 PMID: 16386050
  11. Cloning of yeast gene for trichodermin resistance and ribosomal protein L3.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):238-42 PMID: 7017711
  12. Structural comparison of two nontandemly repeated yeast glyceraldehyde-3-phosphate dehydrogenase genes.
    J Biol Chem. 1980 Mar 25;255(6):2596-605 PMID: 6244283
  13. Localization of dolichyl phosphate- and pyrophosphate-dependent glycosyl transfer reactions in Saccharomyces cerevisiae.
    J Bacteriol. 1979 Aug;139(2):566-72 PMID: 222737
  14. Transformation of yeast by a replicating hybrid plasmid.
    Nature. 1978 Sep 14;275(5676):104-9 PMID: 357984
  15. Multivalent feedback regulation of HMG CoA reductase, a control mechanism coordinating isoprenoid synthesis and cell growth.
    J Lipid Res. 1980 Jul;21(5):505-17 PMID: 6995544
  16. The two methionine adenosyl transferases in Saccharomyces cerevisiae: evidence for the existence of dimeric enzymes.
    Mol Gen Genet. 1981;182(1):65-9 PMID: 7022138
  17. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
    Cell. 1982 Jan;28(1):145-54 PMID: 7039847
  18. Transformation of yeast.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
  19. Isolation and characterisation of a yeast chromosomal replicator.
    Nature. 1979 Nov 1;282(5734):39-43 PMID: 388229
  20. Metabolism of sterols in yeast.
    CRC Crit Rev Microbiol. 1978;6(4):301-41 PMID: 365459
  21. A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts.
    J Bacteriol. 1946 Sep;52(3):293-301 PMID: 16561177
  22. Tunicamycin inhibition of polyisoprenyl N-acetylglucosaminyl pyrophosphate formation in calf-liver microsomes.
    Biochem Biophys Res Commun. 1975 Jul 8;65(1):248-57 PMID: 167767
  23. Analogues of methionine as substrates and inhibitors of the methionine adenosyltransferase reaction. Deductions concerning the conformation of methionine.
    Mol Pharmacol. 1970 Sep;6(5):481-99 PMID: 4918253
  24. Molecular genetics of yeast.
    Annu Rev Biochem. 1980;49:845-76 PMID: 6996573
  25. Segmental aneuploidy and enzyme activity as a method for cytogenetic localization in drosophila melanogaster.
    Genetics. 1974 Feb;76(2):301-9 PMID: 4150789
  26. Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor.
    Cell. 1982 Oct;30(3):933-43 PMID: 6754095
  27. A simple method for the isolation and purification of total lipides from animal tissues.
    J Biol Chem. 1957 May;226(1):497-509 PMID: 13428781
  28. Functional expression of cloned yeast DNA in Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):487-91 PMID: 322128
  29. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  30. Two asparagine synthetases in Saccharomyces cerevisiae.
    Eur J Biochem. 1980 Jul;108(2):373-7 PMID: 6105958
  31. Segmental aneuploidy and the genetic gross structure of the Drosophila genome.
    Genetics. 1972 May;71(1):157-84 PMID: 4624779
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.22.6750
Published
1983-11-00
Pages
6750-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390063
Subset
IM
Grants
NIGMS NIH HHS · 5F32 GM07701-03 · United States
NIGMS NIH HHS · GM-7276 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.22.6750 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2