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PMID: 6318043 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genetic complementation of Agrobacterium tumefaciens Ti plasmid mutants in the virulence region.

Molecular & general genetics : MGG ·Vol. 193 ·No. 1 ·1984-00-00 ·Pages 1-7

Lundquist RC, Close TJ, Kado CI

Abstract

Mutants with Tn5 insertions in the vir region of the Agrobacterium tumefaciens TiC58 plasmid are unable to form crown-gall tumors. Complementation tests of these vir region mutants were carried out by constructing merodiploids in a recombination-deficient strain. Each merodiploid possessed a mutant TiC58 plasmid and a recombinant plasmid containing either the homologous wild-type DNA region or the homologous region containing a second Tn5 insertion. The analysis identified six complementation groups. Mutations in one of these complementation groups were not complemented in trans and represent a cis-dominant locus. The mutation in one complementation group showed variation in host range.

MeSH Terms
Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics Genetic Complementation Test Mutation Plant Tumors/microbiology Plasmids Rhizobium/genetics,pathogenicity Species Specificity Virulence
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lundquist R C
Close T J
Kado C I
References (39)
39 references, click to expand
  1. Control of Tn5 transposition in Escherichia coli is mediated by protein from the right repeat.
    Cell. 1982 Oct;30(3):873-82 PMID: 6291786
  2. The functional organization of the nopaline A. tumefaciens plasmid pTiC58.
    Plasmid. 1980 Mar;3(2):212-30 PMID: 6100894
  3. Polarity of Tn5 insertion mutations in Escherichia coli.
    J Bacteriol. 1980 May;142(2):439-46 PMID: 6247321
  4. Regions of homology between tumorigenic plasmids from Agrobacterium rhizogenes and Agrobacterium tumefaciens.
    Plasmid. 1982 Jan;7(1):45-51 PMID: 7089090
  5. Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mapping.
    Plasmid. 1980 Mar;3(2):193-211 PMID: 6100893
  6. Host range conferred by the virulence-specifying plasmid of Agrobacterium tumefaciens.
    J Bacteriol. 1979 Aug;139(2):591-6 PMID: 457613
  7. Replicon fusions promoted by the inverted repeats of Tn5. The right repeat is an insertion sequence.
    J Mol Biol. 1981 Jul 25;150(1):15-32 PMID: 6271973
  8. Relationship of plasmids responsible for hairy root and crown gall tumorigenicity.
    J Bacteriol. 1980 Nov;144(2):710-20 PMID: 7430069
  9. Grown gall plant tumors of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octopine Ti plasmids; analysis of T-DNA functions.
    Gene. 1981 Jun-Jul;14(1-2):33-50 PMID: 6266929
  10. Complementation analysis of Agrobacterium tumefaciens Ti plasmid mutations affecting oncogenicity.
    J Bacteriol. 1982 Apr;150(1):327-31 PMID: 7061395
  11. Construction and application of R prime plasmids, carrying different segments of an octopine Ti plasmid from Agrobacterium tumefaciens, for complementation of vir genes.
    Plasmid. 1982 Mar;7(2):107-18 PMID: 6281831
  12. Relationship between Nif plasmids of fast-growing Rhizobium species and Ti plasmids of Agrobacterium tumefaciens.
    J Bacteriol. 1982 Mar;149(3):1129-34 PMID: 7061382
  13. Transfection and transformation of Agrobacterium tumefaciens.
    Mol Gen Genet. 1978 Jul 11;163(2):181-7 PMID: 355847
  14. Characteristics of Ti plasmids from broad-host-range and ecologically specific biotype 2 and 3 strains of Agrobacterium tumefaciens.
    J Bacteriol. 1982 Jul;151(1):343-50 PMID: 7085561
  15. Genetic map of the crown gall suppressive IncW plasmid pSa.
    Mol Gen Genet. 1982;186(1):10-5 PMID: 6287164
  16. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.
    Gene. 1977;2(2):75-93 PMID: 344136
  17. Isolation of a recombination deficient Agrobacterium tumefaciens mutant.
    Mol Gen Genet. 1979 Jun 7;173(2):171-5 PMID: 290851
  18. Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism.
    J Bacteriol. 1980 Nov;144(2):732-43 PMID: 6253441
  19. Transfer of RP4::mu plasmids to Agrobacterium tumefaciens.
    Plasmid. 1978 Sep;1(4):446-55 PMID: 372975
  20. Rapid procedure for detection and isolation of large and small plasmids.
    J Bacteriol. 1981 Mar;145(3):1365-73 PMID: 7009583
  21. The control region of the F sex factor DNA transfer cistrons: physical mapping by deletion analysis.
    Mol Gen Genet. 1979 Jan 16;169(1):49-57 PMID: 374998
  22. Physical mapping of DNA base sequence homologies between an octopine and a nopaline Ti plasmid of Agrobacterium tumefaciens.
    J Mol Biol. 1981 Oct 25;152(2):183-208 PMID: 6276566
  23. Characterisation of an in vivo system for nicking at the origin of conjugal DNA transfer of the sex factor F.
    J Mol Biol. 1980 Jan 15;136(2):129-50 PMID: 6154798
  24. Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis.
    Cell. 1977 Jun;11(2):263-71 PMID: 890735
  25. Tn10 transposase acts preferentially on nearby transposon ends in vivo.
    Cell. 1983 Mar;32(3):799-807 PMID: 6299577
  26. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  27. Genetic analysis of T-DNA transcripts in nopaline crown galls.
    Cell. 1983 Apr;32(4):1057-67 PMID: 6839358
  28. Agrobacterium tumefaciens mutants affected in attachment to plant cells.
    J Bacteriol. 1982 Dec;152(3):1265-75 PMID: 6292165
  29. Genetic Identification of functions of TL-DNA transcripts in octopine crown galls.
    EMBO J. 1982;1(1):147-52 PMID: 16453404
  30. Multiple mutations in the T region of the Agrobacterium tumefaciens tumor-inducing plasmid.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1660-4 PMID: 6300864
  31. Indoleacetic acid complementation and its relation to host range specifying genes on the Ti plasmid of Agrobacterium tumefaciens.
    Mol Gen Genet. 1982;188(3):425-32 PMID: 6962315
  32. Mutational analysis of the virulence region of an Agrobacterium tumefaciens Ti plasmid.
    J Bacteriol. 1983 Feb;153(2):878-83 PMID: 6296058
  33. Units of genetic expression in the virulence region of a plant tumor-inducing plasmid of Agrobacterium tumefaciens.
    Mol Gen Genet. 1982;188(3):418-24 PMID: 6298572
  34. Characterization of Tn904 insertions in octopine Ti plasmid mutants of Agrobacterium tumefaciens.
    J Bacteriol. 1980 Oct;144(1):82-91 PMID: 6252198
  35. A general method for site-directed mutagenesis in prokaryotes.
    Nature. 1981 Jan 1;289(5793):85-8 PMID: 6256652
  36. Agrobacterium Ti plasmid indoleacetic acid gene is required for crown gall oncogenesis.
    Proc Natl Acad Sci U S A. 1982 May;79(9):2812-6 PMID: 16593184
  37. Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis.
    Cell. 1981 Nov;27(1 Pt 2):143-53 PMID: 6276020
  38. Internal organization, boundaries and integration of Ti-plasmid DNA in nopaline grown gall tumours.
    J Mol Biol. 1980 Dec 15;144(3):353-76 PMID: 7253020
  39. The rapid purification of T4 DNA ligase from a lambda T4 lig lysogen.
    J Biol Chem. 1980 Feb 10;255(3):813-5 PMID: 6985906
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327406
Published
1984-00-00
Pages
1-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NCI NIH HHS · CA-11526 · United States
External Links
PubMed source DOI: 10.1007/BF00327406
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