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PMID: 6318050 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genes for gentamicin-(3)-N-acetyltransferases III and IV: I. Nucleotide sequence of the AAC(3)-IV gene and possible involvement of an IS140 element in its expression.

Molecular & general genetics : MGG ·Vol. 193 ·No. 1 ·1984-00-00 ·Pages 179-87

Bräu B, Pilz U, Piepersberg W

Abstract

Genes for gentamicin-3-acetyltransferases [ACC(3)] of types III and IV have been cloned from various R-plasmids. In two R-plasmids, pWP14a (AAC(3)-III) and pWP7b [AAC(3)-IV], resistance genes have been found directly adjacent to a single copy of an IS element, IS140. Nucleotide sequence determination of the AAC(3)-IV gene from plasmid pWP7b and of part of IS140 from three different sources suggested that the -35 region of the AAC(3)-IV promoter was part of the IS element. A similarly built-up promoter was found in pWP14a. It was found also, that a hygromycin B phosphotransferase was expressed from a locus neighbouring the AAC(3)-IV gene in pWP7b which was under the control of the same promoter. In two other R-plasmids, pWP113a and pWP116a, the AAC(3)-III gene was found in different genetic environments, namely close to Tn3-like structures.

MeSH Terms
Acetyltransferases/genetics Base Sequence DNA Restriction Enzymes DNA Transposable Elements Drug Resistance, Microbial Escherichia coli/enzymology,genetics Genes Genes, Bacterial Nucleic Acid Hybridization Plasmids Recombination, Genetic Transcription, Genetic
Chemicals
DNA Transposable Elements Acetyltransferases gentamicin 3-acetyltransferase DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bräu B
Pilz U
Piepersberg W
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37 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327434
Published
1984-00-00
Pages
179-87
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X01385
External Links
PubMed source DOI: 10.1007/BF00327434
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