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PMID: 6318433 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Antigenic relationships of murine coronaviruses: analysis using monoclonal antibodies to JHM (MHV-4) virus.

Virology ·Vol. 131 ·No. 2 ·1983-12-00 ·Pages 296-307

Fleming JO, Stohlman SA, Harmon RC, Lai MM, Frelinger JA, Weiner LP

Abstract

Monoclonal antibodies were produced to JHMV-DL, a neurotropic member of the mouse hepatitis virus (MHV) or murine coronavirus group. Of 23 antibodies isolated, 10 were specific for the major envelope glycoprotein, gp180/90, 10 for the nucleocapsid protein, pp60, and 3 for the minor envelope glycoprotein, gp25. Eleven different MHV isolates were used in antibody binding assays to study antigenic relationships among the viruses. Each MHV isolate tested had a unique pattern of antibody binding, indicating that each is a distinct strain. Conservation of JHMV-DL antigenic determinants varied among the three proteins, with pp60 showing intermediate conservation, gp180/90 little conservation, and gp25 marked conservation in the different MHV strains. Monoclonal antibodies to pp60 proved most useful in delineating antigenic relationships among MHV strains. These antigenic groups correlated with pathogenic types, indicating that pp60 may be one of the gene products which mediates the distinct disease patterns manifested by different murine coronaviruses.

MeSH Terms
Animals Antibodies, Monoclonal/analysis,isolation & purification Antigens, Viral/analysis Binding Sites, Antibody Immune Sera/isolation & purification Mice Mice, Inbred C57BL Murine hepatitis virus/immunology Neutralization Tests Precipitin Tests Radioimmunoassay Viral Proteins/immunology Viral Structural Proteins Virus Cultivation
Chemicals
Antibodies, Monoclonal Antigens, Viral Immune Sera Viral Proteins Viral Structural Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Fleming J O
Stohlman S A
Harmon R C
Lai M M
Frelinger J A
Weiner L P
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Article Info
Journal
Virology
Abbr.
Virology
ISSN
0042-6822
DOI
10.1016/0042-6822(83)90498-1
Published
1983-12-00
Pages
296-307
Language
English
Region
United States
NLM ID
0110674
PMCID
PMC7131482
Subset
IM
Grants
NCI NIH HHS · CA 22662 · United States
NINDS NIH HHS · NS 18146 · United States
NINDS NIH HHS · NS-07149 · United States
External Links
PubMed source DOI: 10.1016/0042-6822(83)90498-1 PMC Full Text View journal details
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