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PMID: 631889 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Protein-free and low-protein media for the cultivation of Leptospira.

Infection and immunity ·Vol. 19 ·No. 2 ·1978-02-00 ·Pages 562-9

Bey RF, Johnson RC

Abstract

A protein-free medium composed of charcoal-detoxified Tweens (polysorbates), vitamins B12 and B1, inorganic salts, and organic buffer is described that supports the growth and subculture of pathogenic and saprophytic Leptospira. Growth was initiated from small inocula, and cell densities of 10(9) organisms per ml were attained. Antigenicity and immunogenicity of Leptospira cultivated in this medium were similar to those of cells cultivated in serum-containing media. The protein-free medium was converted to a low-protein medium by the addition of 0.1% bovine serum albumin.

MeSH Terms
Antigens, Bacterial Culture Media Leptospira/growth & development,immunology,pathogenicity Polysorbates Serum Albumin, Bovine Species Specificity Virulence
Chemicals
Antigens, Bacterial Culture Media Polysorbates Serum Albumin, Bovine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bey R F
Johnson R C
References (24)
24 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.19.2.562-569.1978
Published
1978-02-00
Pages
562-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC414120
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.19.2.562-569.1978 PMC Full Text View journal details
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