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PMID: 6319431 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Interaction between major histocompatibility complex antigens and epidermal growth factor receptors on human cells.

The Journal of cell biology ·Vol. 98 ·No. 2 ·1984-02-00 ·Pages 725-31

Schreiber AB, Schlessinger J, Edidin M

Abstract

It has been suggested that products of the major histocompatibility complex, the MHC, of vertebrates function in many processes of recognition and ligand binding at the cell surface. Here we show that binding of polyclonal and monoclonal antibodies against human MHC antigens, HLA, reduced the binding of epidermal growth factor (EGF) to its membrane receptors on A-431 tumor cells and on normal human fibroblasts. Binding of EGF at 37 degrees C similarly inhibited the binding of Fab fragments and intact Ig anti-HLA to human cells. The inhibitory effect of anti-HLA antibodies was rapid and dependent upon temperature and antibody concentration and valence. Fluorescence microscopy qualitatively confirmed the binding data and showed that MHC antigens and EGF-receptors do not co-cluster in the membrane.

MeSH Terms
Animals Antibodies Antigen-Antibody Complex Carcinoma, Squamous Cell Cell Line Cell Membrane/immunology,metabolism Epidermal Growth Factor/metabolism ErbB Receptors HLA Antigens/immunology Humans Major Histocompatibility Complex Male Mice Microscopy, Fluorescence Receptors, Cell Surface/metabolism
Chemicals
Antibodies Antigen-Antibody Complex HLA Antigens Receptors, Cell Surface Epidermal Growth Factor ErbB Receptors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schreiber A B
Schlessinger J
Edidin M
References (36)
36 references, click to expand
  1. Characterization by electrophoresis of epidermal growth factor stimulated phosphorylation using A-431 membranes.
    Biochemistry. 1980 Apr 1;19(7):1524-8 PMID: 6248106
  2. Monoclonal antibodies against receptor for epidermal growth factor induce early and delayed effects of epidermal growth factor.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7535-9 PMID: 6278478
  3. Tumor-promoting phorbol esters inhibit binding of epidermal growth factor to cellular receptors.
    Science. 1978 Oct 20;202(4365):313-5 PMID: 308698
  4. An investigation of sites that bind human somatotropin (growth hormone) in the liver of the pregnant rabbit.
    Biochem J. 1981 Sep 15;198(3):605-14 PMID: 6275854
  5. Dissociation and exchange of the beta 2-microglobulin subunit of HLA-A and HLA-B antigens.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5834-8 PMID: 93282
  6. Interactions between the receptors for platelet-derived growth factor and epidermal growth factor.
    J Cell Biol. 1983 Mar;96(3):679-83 PMID: 6300138
  7. Vasopressin inhibition of epidermal growth factor binding to cultured mouse cells.
    J Biol Chem. 1981 Jan 25;256(2):716-22 PMID: 6256365
  8. Stimulation of DNA synthesis in quiescent cultured cells: exogenous agents, internal signals, and early events.
    Curr Top Cell Regul. 1980;17:59-88 PMID: 6254731
  9. Epidermal growth factor and a new derivative. Rapid isolation procedures and biological and chemical characterization.
    J Biol Chem. 1972 Dec 10;247(23):7609-11 PMID: 4636326
  10. Rotational diffusion of epidermal growth factor complexed to cell surface receptors reflects rapid microaggregation and endocytosis of occupied receptors.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6981-5 PMID: 6273899
  11. Role of MHC gene products in immune regulation.
    Science. 1981 Jun 12;212(4500):1229-38 PMID: 6165083
  12. Direct visualization of the binding and internalization of a ferritin conjugate of epidermal growth factor in human carcinoma cells A-431.
    J Cell Biol. 1979 May;81(2):382-95 PMID: 313931
  13. Studies in histocompatibility.
    Science. 1981 Jul 10;213(4504):172-8 PMID: 7017931
  14. Identity of human epidermal growth factor (EGF) receptor with glycoprotein SA-7: evidence for differential phosphorylation of the two components of the EGF receptor from A431 cells.
    Proc Natl Acad Sci U S A. 1982 Aug;79(16):5026-30 PMID: 6289330
  15. Immunoglobulin binding by mouse intestinal epithelial cell receptors.
    J Immunol. 1976 Aug;117(2):587-93 PMID: 950463
  16. The kinetics of antibody binding to membrane antigens in solution and at the cell surface.
    Biochem J. 1980 Apr 1;187(1):1-20 PMID: 6967725
  17. Heavy chain of HLA-A and HLA-B antigens is conformationally labile: a possible role for beta 2-microglobulin.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3844-8 PMID: 91170
  18. Preparation of iodine-131 labelled human growth hormone of high specific activity.
    Nature. 1962 May 5;194:495-6 PMID: 14450081
  19. The H-2 locus of the mouse: observations and speculations concerning its comparative genetics and its polymorphism.
    Folia Biol (Praha). 1968;14(5):335-58 PMID: 4881822
  20. Influence of the mouse major histocompatibility complex, H-2, on liver adenylate cyclase activity and on glucagon binding to liver cell membranes.
    Biochemistry. 1980 Jan 8;19(1):49-54 PMID: 6243475
  21. Possible roles of compound membrane receptors in the immune system.
    Ann Immunol (Paris). 1983 Jul-Aug;134D(1):85-92 PMID: 6354069
  22. Interaction of wheat germ agglutinin and concanavalin A with isolated fat cells.
    Biochemistry. 1973 Mar 27;12(7):1312-23 PMID: 4696755
  23. Is the MHC a general self-recognition system playing a major unifying role in an organism?
    Hum Immunol. 1980 Jul;1(1):5-17 PMID: 6455393
  24. Direct visualization of binding, aggregation, and internalization of insulin and epidermal growth factor on living fibroblastic cells.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2659-63 PMID: 307249
  25. Intercellular adhesiveness of H-2 identical and H-2 disparate cells.
    J Immunogenet. 1978 Feb;5(1):41-7 PMID: 641358
  26. Association of mouse liver adenosine 3':5'-cyclic monophosphate (cyclic AMP) levels with histocompatibility-2 genotype.
    Proc Natl Acad Sci U S A. 1975 Jul;72(7):2644-8 PMID: 170610
  27. Primary amines do not prevent the endocytosis of epidermal growth factor into 3T3 fibroblasts.
    Biochim Biophys Acta. 1981 May 5;674(2):188-203 PMID: 6263349
  28. Protochordate allorecognition is controlled by a MHC-like gene system.
    Nature. 1982 Feb 11;295(5849):499-502 PMID: 7057909
  29. Enhanced expression of histocompatibility antigens on interferon-treated mouse embryonic fibroblasts.
    Proc Soc Exp Biol Med. 1978 Mar;157(3):456-60 PMID: 634988
  30. Cell membrane antigens recognized by anti-viral and anti-trinitrophenyl cytotoxic T lymphocytes.
    Immunol Rev. 1981;58:73-94 PMID: 6273294
  31. Lateral diffusion of rhodopsin in photoreceptor cells measured by fluorescence photobleaching and recovery.
    Biophys J. 1981 Feb;33(2):225-32 PMID: 6971659
  32. Epidermal growth factor.
    Annu Rev Biochem. 1979;48:193-216 PMID: 382984
  33. Effect of the H-2 gene complex rates of fibroblast intercellular adhesion.
    J Cell Biol. 1978 May;77(2):377-88 PMID: 565780
  34. The original function of MHC antigens as the general plasma membrane anchorage site of organogenesis-directing proteins.
    Immunol Rev. 1977 Jan;33:59-69 PMID: 66186
  35. Biologically active phorbol esters specifically alter affinity of epidermal growth factor membrane receptors.
    Nature. 1979 May 31;279(5712):387-91 PMID: 16068160
  36. Conjugates of immunoglobulin G with different fluorochromes. I. Characterization by anionic-exchange chromatography.
    Scand J Immunol. 1973;2(3):273-90 PMID: 4797751
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.98.2.725
Published
1984-02-00
Pages
725-31
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113088
Subset
IM
Grants
NIAID NIH HHS · AI-14584 · United States
NCI NIH HHS · CA-25820 · United States
External Links
PubMed source DOI: 10.1083/jcb.98.2.725 PMC Full Text View journal details
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