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PMID: 6319530 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Epstein-Barr virus susceptibility of normal human B lymphocyte populations.

The Journal of experimental medicine ·Vol. 159 ·No. 1 ·1984-01-01 ·Pages 208-20

Aman P, Ehlin-Henriksson B, Klein G

Abstract

Human blood and tonsil B lymphocytes were fractionated on density gradients and tested for virus binding and penetration into the cells. Epstein-Barr Virus (EBV) transformation was detected by immunofluorescence staining for EBV-determined nuclear antigen (EBNA). EBV bound to and penetrated all B cell populations, but only the high density populations were transformed. Activated B lymphocytes were found in the low density fractions and these cells were resistant to EBV infection. Infected and noninfected B lymphocytes were density-analyzed during in vitro culture. A spontaneous, not virus-induced, density decrease was found to precede the production of EBNA. Cells remaining at high density never expressed EBNA. The results suggest that EBV can transform only small resting B lymphocytes and that a virus-independent activation of the infected cells induces the EBNA production and transformation.

MeSH Terms
Antigens, Viral/analysis B-Lymphocytes/classification,immunology Cell Count Cell Line Cell Separation Cell Transformation, Viral Disease Susceptibility Epstein-Barr Virus Nuclear Antigens Herpesviridae Infections/immunology Herpesvirus 4, Human/immunology Humans Lymphocyte Activation Receptors, Virus/analysis Rosette Formation
Chemicals
Antigens, Viral Epstein-Barr Virus Nuclear Antigens Receptors, Virus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Aman P
Ehlin-Henriksson B
Klein G
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34 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.159.1.208
Published
1984-01-01
Pages
208-20
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187189
Subset
IM
Grants
NCI NIH HHS · 1R01 CA 30264-01 · United States
External Links
PubMed source DOI: 10.1084/jem.159.1.208 PMC Full Text View journal details
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