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PMID: 6320126 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structure of the chloroplast gene for the precursor of the Mr 32,000 photosystem II protein from mustard (Sinapis alba L.).

Nucleic acids research ·Vol. 12 ·No. 2 ·1984-01-25 ·Pages 945-58

Link G, Langridge U

Abstract

The nucleotide sequence of the mustard chloroplast gene for the precursor of the Mr 32,000 photosystem II protein is presented. A comparison with the corresponding genes from spinach and Nicotiana debneyi (14) reveals less than 5% nucleotide divergence in the coding region. The derived protein of mustard differs from the corresponding proteins by three amino acid positions at the C-terminus. We have defined the presumed transcription start and termination sites of the mustard gene. Upstream from the start site are sequences typical of a prokaryotic promoter and, also, a sequence that resembles the eukaryotic 'TATA' box. A search for intrastrand base pairing revealed stem-loop secondary structure at the transcription start and termination sites and in the region preceding the presumed promoter. This latter region is a 69-base-pair sequence element unique to the 5'flanking sequence of the mustard gene.

MeSH Terms
Base Sequence Chloroplasts/metabolism DNA Restriction Enzymes Genes Molecular Weight Mustard Plant/metabolism Nucleic Acid Conformation Photosynthesis Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins/genetics Plants/metabolism Plants, Medicinal Plants, Toxic Protein Precursors/genetics Species Specificity Tobacco/metabolism Transcription, Genetic
Chemicals
Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins Protein Precursors DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Link G
Langridge U
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30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.2.945
Published
1984-01-25
Pages
945-58
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318547
Subset
IM
Databases
GENBANK
X00340
External Links
PubMed source DOI: 10.1093/nar/12.2.945 PMC Full Text View journal details
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