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PMID: 6320162 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Amphipathic analysis and possible formation of the ion channel in an acetylcholine receptor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 1 ·1984-01-00 ·Pages 155-9

Finer-Moore J, Stroud RM

Abstract

Fourier analysis of the hydrophobicities of the acetylcholine receptor subunit sequences reveals regions of amphipathic secondary structure. Prediction of a consensus secondary structure based on this analysis and on an empirical prediction method leads to a testable hypothesis about how the ion channel is formed and might function. Knowledge of the three-dimensional structure of acetylcholine receptors is consistent with features of the model proposed and provides some constraints.

MeSH Terms
Amino Acid Sequence Animals Calorimetry Ion Channels/metabolism Kinetics Macromolecular Substances Mathematics Models, Biological Protein Conformation Receptors, Cholinergic/metabolism
Chemicals
Ion Channels Macromolecular Substances Receptors, Cholinergic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Finer-Moore J
Stroud R M
References (34)
34 references, click to expand
  1. Biochemical properties of acteylcholine receptor subunits from Torpedo californica.
    Biochemistry. 1979 Oct 16;18(21):4465-70 PMID: 497150
  2. Primary structure of alpha-subunit precursor of Torpedo californica acetylcholine receptor deduced from cDNA sequence.
    Nature. 1982 Oct 28;299(5886):793-7 PMID: 6182472
  3. Mechanisms for the incorporation of proteins in membranes and organelles.
    J Cell Biol. 1982 Jan;92(1):1-22 PMID: 7035466
  4. Structural models of the nicotinic acetylcholine receptor and its toxin-binding sites.
    Cell Mol Neurobiol. 1981 Sep;1(3):231-58 PMID: 7346169
  5. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  6. Structure and function of an acetylcholine receptor.
    Biophys J. 1982 Jan;37(1):371-83 PMID: 7055628
  7. Inhibition of glycosylation with tunicamycin blocks assembly of newly synthesized acetylcholine receptor subunits in muscle cells.
    J Biol Chem. 1982 Mar 10;257(5):2694-701 PMID: 7061443
  8. Complete mRNA coding sequence of the acetylcholine binding alpha-subunit of Torpedo marmorata acetylcholine receptor: a model for the transmembrane organization of the polypeptide chain.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):2067-71 PMID: 6572962
  9. Acetylcholine receptor channel ionic selectivity: ions experience an aqueous environment.
    Proc Natl Acad Sci U S A. 1983 Oct;80(19):6110-3 PMID: 6310616
  10. Acetylcholine receptor: complex of homologous subunits.
    Science. 1980 Jun 27;208(4451):1454-6 PMID: 7384786
  11. The 14-fold periodicity in alpha-tropomyosin and the interaction with actin.
    J Mol Biol. 1976 May 15;103(2):271-98 PMID: 950663
  12. Acetylcholine receptor subunits transit a precursor pool before acquiring alpha-bungarotoxin binding activity.
    J Biol Chem. 1981 Apr 25;256(8):3605-8 PMID: 7217046
  13. Nucleotide and deduced amino acid sequences of Torpedo californica acetylcholine receptor gamma subunit.
    Proc Natl Acad Sci U S A. 1983 Feb;80(4):1111-5 PMID: 6573658
  14. The molecular cloning and characterisation of cDNA coding for the alpha subunit of the acetylcholine receptor.
    Nucleic Acids Res. 1982 Oct 11;10(19):5809-22 PMID: 6183641
  15. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
    J Mol Biol. 1978 Mar 25;120(1):97-120 PMID: 642007
  16. Tests for comparing related amino-acid sequences. Cytochrome c and cytochrome c 551 .
    J Mol Biol. 1971 Oct 28;61(2):409-24 PMID: 5167087
  17. The beta bulge: a common small unit of nonrepetitive protein structure.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2574-8 PMID: 275827
  18. Conformational and geometrical properties of beta-sheets in proteins. II. Antiparallel and mixed beta-sheets.
    J Mol Biol. 1981 Feb 15;146(1):119-41 PMID: 7265226
  19. Primary structures of beta- and delta-subunit precursors of Torpedo californica acetylcholine receptor deduced from cDNA sequences.
    Nature. 1983 Jan 20;301(5897):251-5 PMID: 6687403
  20. Structural homology of Torpedo californica acetylcholine receptor subunits.
    Nature. 1983 Apr 7;302(5908):528-32 PMID: 6188060
  21. Periodic features in the amino acid sequence of nematode myosin rod.
    J Mol Biol. 1983 Mar 15;164(4):605-26 PMID: 6341606
  22. The permeability of the endplate channel to organic cations in frog muscle.
    J Gen Physiol. 1980 May;75(5):469-92 PMID: 6247422
  23. Permeability of the endplate membrane activated by acetylcholine to some organic cations.
    J Neurobiol. 1977 Mar;8(2):173-84 PMID: 300788
  24. Effects of methyl- and ethyl-derivatives of NH4 on the neuromuscular junction.
    Jpn J Physiol. 1959 Jun 25;9(2):130-42 PMID: 13672685
  25. Molecular weight in detergent solution of acetylcholine receptor from Torpedo californica.
    Biochemistry. 1978 May 30;17(11):2035-8 PMID: 667008
  26. Ion-concentration dependence of the reversal potential and the single channel conductance of ion channels at the frog neuromuscular junction.
    J Physiol. 1979 Jan;286:417-45 PMID: 312319
  27. beta-Sheet topology and the relatedness of proteins.
    Nature. 1977 Aug 11;268(5620):495-500 PMID: 329147
  28. Immunospecific identification and three-dimensional structure of a membrane-bound acetylcholine receptor from Torpedo californica.
    J Mol Biol. 1979 Mar 5;128(3):319-34 PMID: 439138
  29. Structural studies of a membrane-bound acetylcholine receptor from Torpedo californica.
    J Mol Biol. 1977 Nov;116(4):635-59 PMID: 563472
  30. Partial tertiary structure assignment for the acetylcholine receptor on the basis of the hydrophobicity of amino acid sequences and channel location using single group rotation theory.
    Biochem Biophys Res Commun. 1983 Mar 29;111(3):1022-6 PMID: 6301478
  31. Partial tertiary structure assignments for the beta-, gamma- and delta-subunits of the acetylcholine receptor on the basis of the hydrophobicity of amino acid sequences and channel location using single group rotation theory.
    FEBS Lett. 1983 May 8;155(2):245-7 PMID: 6303846
  32. On the conformation of the acetylcholine receptor protein from Torpedo nobiliana.
    FEBS Lett. 1974 Sep 1;45(1):145-9 PMID: 4414912
  33. Selectivity of cations and nonelectrolytes for acetylcholine-activated channels in cultured muscle cells.
    J Gen Physiol. 1978 Apr;71(4):397-410 PMID: 566305
  34. In vitro synthesis, glycosylation, and membrane insertion of the four subunits of Torpedo acetylcholine receptor.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5598-602 PMID: 6946496
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.1.155
Published
1984-01-00
Pages
155-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344629
Subset
IM
Grants
NIGMS NIH HHS · GM 24485 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.1.155 PMC Full Text View journal details
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